A Transgenic System for Rapid Magnetic Enrichment of Rare Embryonic Cells.

Szczegóły
Abstrakt

Tytuł:: A Transgenic System for Rapid Magnetic Enrichment of Rare Embryonic Cells.
Autorzy:: Wattrus SJ; Stem Cell Program and Division of Hematology/Oncology, Boston Children's Hospital and Dana Farber Cancer Institute, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts, USA.; Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts, USA.
Zon LI; Stem Cell Program and Division of Hematology/Oncology, Boston Children's Hospital and Dana Farber Cancer Institute, Howard Hughes Medical Institute, Harvard Medical School, Boston, Massachusetts, USA.; Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts, USA.
Źródło:: Zebrafish [Zebrafish] 2020 Oct; Vol. 17 (5), pp. 354-357. Date of Electronic Publication: 2020 Jul 29.
Typ publikacji:: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: Larchmont, NY : Mary Ann Liebert, Inc., c2004-
MeSH Terms:: Embryo, Nonmammalian/*embryology
Embryology/*methods
High-Throughput Nucleotide Sequencing/*instrumentation
Zebrafish/*embryology
Animals ; Animals, Genetically Modified/embryology ; Embryonic Development ; Endothelial Cells/chemistry ; Hematopoietic Stem Cells/chemistry ; Humans ; Magnetic Phenomena ; Single-Cell Analysis/instrumentation
References:: Dev Biol. 2007 Apr 15;304(2):735-44. (PMID: 17306248)
Cytometry. 1990;11(2):231-8. (PMID: 1690625)
Cell. 2015 Jan 15;160(1-2):241-52. (PMID: 25594182)
Nat Commun. 2015 Sep 28;6:8375. (PMID: 26411530)
Sci Rep. 2019 Jan 18;9(1):227. (PMID: 30659223)
Cell. 2015 May 21;161(5):1187-1201. (PMID: 26000487)
F1000Res. 2016 Aug 31;5:2122. (PMID: 27909575)
Genome Biol. 2016 Feb 17;17:29. (PMID: 26887813)
Grant Information:: U54 DK110805 United States DK NIDDK NIH HHS; R01 HL144780 United States HL NHLBI NIH HHS; United States HHMI Howard Hughes Medical Institute; F31 HL149154 United States HL NHLBI NIH HHS; P01 HL032262 United States HL NHLBI NIH HHS; P01 HL131477 United States HL NHLBI NIH HHS; RC2 DK120535 United States DK NIDDK NIH HHS; R24 OD017870 United States OD NIH HHS; R24 DK092760 United States DK NIDDK NIH HHS
Contributed Indexing:: Keywords: enrichment; hematopoiesis; magnetic; single cell; tool
Entry Date(s):: Date Created: 20200805 Date Completed: 20210412 Latest Revision: 20211002
Update Code:: 20240105
PubMed Central ID:: PMC7578180
DOI:: 10.1089/zeb.2020.1904
PMID:: 32749922
: Czasopismo naukowe

Collecting large numbers of rare cells for high-throughput molecular analysis remains a technical challenge, primarily due to limitations in existing technologies. In developmental biology this has impeded single-cell analysis of primordial organs, which derive from few cells. In this study, we share novel transgenic lines for rapid cell enrichment from zebrafish embryos using human surface antigens for immunological binding and magnetic sorting. As proof of principle, we tagged, enriched, and performed single-cell RNA sequencing on nascent hematopoietic stem/progenitor cells and endothelial cells from early embryos. Our method is a quick, efficient, and cost-effective approach to a previously intractable problem.

Informacja