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Tytuł:
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Identification and characterization of PaGL1-like genes from Platanus acerifolia related to the regulation of trichomes.
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Autorzy:
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Zhang Y; Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.; Key Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Wuhan, 430070, People's Republic of China.
Shao C; Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.; Key Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Wuhan, 430070, People's Republic of China.
Bao Z; Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.; Key Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Wuhan, 430070, People's Republic of China.
Cai F; Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.; Key Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Wuhan, 430070, People's Republic of China.
Zhu H; Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.; Key Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Wuhan, 430070, People's Republic of China.
Zhang J; Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China. .; Key Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Wuhan, 430070, People's Republic of China. .
Bao M; Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China. .; Key Laboratory of Urban Agriculture in Central China, Ministry of Agriculture and Rural Affairs, Wuhan, 430070, People's Republic of China. .
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Źródło:
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Plant molecular biology [Plant Mol Biol] 2020 Oct; Vol. 104 (3), pp. 235-248. Date of Electronic Publication: 2020 Aug 05.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Dordrecht : Kluwer Academic
Original Publication: The Hague ; Boston : Martinus Nijhoff/Dr. W. Junk, 1981-
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MeSH Terms:
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DNA-Binding Proteins/*genetics
DNA-Binding Proteins/*metabolism
Plant Proteins/*genetics
Plant Proteins/*metabolism
Tracheophyta/*genetics
Trichomes/*metabolism
Arabidopsis/genetics ; Arabidopsis Proteins/genetics ; Arabidopsis Proteins/metabolism ; Basic Helix-Loop-Helix Transcription Factors ; Gene Expression Regulation, Plant ; Homeodomain Proteins/genetics ; Phylogeny ; Plant Leaves/genetics ; Plants, Genetically Modified ; Sequence Alignment ; Tracheophyta/metabolism ; Transcription Factors/genetics ; Transcriptome ; Trichomes/growth & development
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Grant Information:
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31672187 National Natural Science Foundation of China; 31570696 Natural Science Foundation of Shanghai
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Contributed Indexing:
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Keywords: London plane; Protein interaction; R2R3 MYB transcription factor; Trichome regulation
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Substance Nomenclature:
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0 (Arabidopsis Proteins)
0 (Basic Helix-Loop-Helix Transcription Factors)
0 (DNA-Binding Proteins)
0 (GL2 protein, Arabidopsis)
0 (Homeodomain Proteins)
0 (Plant Proteins)
0 (Transcription Factors)
144417-31-2 (GL1 protein, Arabidopsis)
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Entry Date(s):
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Date Created: 20200807 Date Completed: 20200928 Latest Revision: 20220416
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Update Code:
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20240105
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DOI:
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10.1007/s11103-020-01028-5
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PMID:
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32757127
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Key Message: Two PaGL1-like genes were identified in London plane and functional in Arabidopsis, moreover, may play an important role in the regulation of trichome development in London plane. Trichome development is governed by a complex regulatory network. In Arabidopsis, subgroup 15 of the R2R3 MYB transcription factor family, which includes GLABRA1 (GL1), is involved in trichome development. In this study, we isolated and characterized two PaGL1-like genes from London plane (Platanus acerifolia). Sequence alignment and phylogenetic analysis indicated that these PaGL1-like genes are homologous to AtGL1. Quantitative real-time PCR (qRT-PCR) analysis showed that PaGL1-like1 was expressed in all of the tested organs taken from adult London plane trees, including trichomes, petioles after trichome removal, stems after trichome removal, and leaves after trichome removal, and also in the roots, cotyledons, hypocotyls and true leaves of seedlings. By contrast, the PaGL1-like2 was expressed only in the trichomes and leaves after trichome removal from adult trees, and in the cotyledons and true leaves of seedlings. Overexpression of PaGL1-like genes caused trichome abortion when transferred into wild type Arabidopsis and promoted trichome formation in the gl1 mutant. The expression profiles of some trichome-related genes were changed in transgenic Arabidopsis lines, and yeast two-hybrid analysis indicated that PaGL1-like proteins can directly interact with trichome-related bHLH proteins from both P. acerifolia and Arabidopsis. These results suggest that PaGL1-like genes are functional in Arabidopsis and may play an important role in the regulation of trichome development in London plane.
