Biocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on glass and glass-ceramic materials.

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Abstrakt

Tytuł:: Biocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on glass and glass-ceramic materials.
Autorzy:: de Araújo Lopes JM; Endodontics Section, Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (Unesp), Araçatuba, Brazil.
Benetti F; Endodontics Section, Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (Unesp), Araçatuba, Brazil.; Endodontic Section, Department of Restorative Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Rezende GC; Endodontics Section, Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (Unesp), Araçatuba, Brazil.
Souza MT; Vitreous Materials Laboratory (LaMaV), Department of Materials Engineering, Federal University of São Carlos (UFSCar), São Carlos, Brazil.
Conti LC; Endodontics Section, Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (Unesp), Araçatuba, Brazil.
Ervolino E; Department of Basic Science, School of Dentistry, São Paulo State University (Unesp), Araçatuba, Brazil.
Jacinto RC; Endodontics Section, Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (Unesp), Araçatuba, Brazil.
Zanotto ED; Vitreous Materials Laboratory (LaMaV), Department of Materials Engineering, Federal University of São Carlos (UFSCar), São Carlos, Brazil.
Cintra LTA; Endodontics Section, Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (Unesp), Araçatuba, Brazil.
Źródło:: International endodontic journal [Int Endod J] 2020 Nov; Vol. 53 (11), pp. 1494-1505. Date of Electronic Publication: 2020 Sep 04.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Oxford, Blackwell Scientific Publications.
MeSH Terms:: Anti-Infective Agents*
Calcium Hydroxide*/pharmacology
Animals ; Ceramics ; Enterococcus faecalis ; Pilot Projects ; Rats
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Grant Information:: 455943/2014-1 Conselho Nacional de Desenvolvimento Científico e Tecnológico; 305969/2015-3 Conselho Nacional de Desenvolvimento Científico e Tecnológico
Contributed Indexing:: Keywords: bioactive glass; bioceramics; biocompatibility; biomineralization; osteopontin
Substance Nomenclature:: 0 (Anti-Infective Agents)
PF5DZW74VN (Calcium Hydroxide)
Entry Date(s):: Date Created: 20200809 Date Completed: 20201208 Latest Revision: 20201214
Update Code:: 20240105
DOI:: 10.1111/iej.13382
PMID:: 32767572
: Czasopismo naukowe

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Aim: To evaluate the biocompatibility, induction of mineralization and antimicrobial activity of experimental intracanal pastes based on two glass and glass-ceramic materials. Calcium hydroxide (Ca(OH) 2 ) paste was used as the positive control.
Methodology: The glass-ceramic powder [two-phased Biosilicate (BS-2P)] and F18 bioactive glass were mixed with distilled water (ratio 2 : 1), inserted in polyethylene tubes and implanted in the subcutaneous tissues of 16 rats. Empty tubes were used as negative control. After 7 and 30 days (n = 8), the rats were euthanized for haematoxylin-eosin, von Kossa, polarized light and osteopontin (OPN) immunolabeling analysis. Direct contact tests using a suspension of each paste were performed with Enterococcus faecalis planktonic cells to evaluate antimicrobial activity (24 h of contact), in a pilot study. The number of CFU mL -1 was calculated for each group. The antimicrobial analysis data were submitted to one-way anova and Tukey tests, whilst biocompatibility and immunohistochemical data were submitted to the Kruskal-Wallis and Dunn tests (P < 0.05).
Results: Most specimens of the control, BS-2P and Ca(OH) 2 groups were associated with moderate inflammation seven days following implantation, whilst F18 was associated with moderate to severe inflammation, without differences amongst the groups (P > 0.05). At 30 days, most specimens of control, F18 and BS-2P groups had mild inflammation, whilst Ca(OH) 2 had mild to moderate inflammation; however, no differences were determined amongst the groups (P > 0.05). The fibrous capsule was thick at 7 days, becoming thin at 30 days. All pastes induced von Kossa-positive structures and were birefringent to polarized light. At seven days, the BS-2P group had significantly more OPN immunolabeling compared to the control and Ca(OH) 2 groups (P < 0.05). At 30 days, the F18 group had significantly more OPN immunolabeling compared to the control and Ca(OH) 2 groups (P < 0.05). All pastes reduced the total number of E. faecalis; however, the reduction was only significant when comparing BS-2P and Ca(OH) 2 groups to the control (P < 0.05). Only calcium hydroxide eliminated E. faecalis.
Conclusions: Experimental BS-2P and F18 pastes were biocompatible, stimulated biomineralization and induced significant OPN immunolabeling compared to Ca(OH) 2 . Only the BS-2P paste demonstrated antimicrobial activity comparable to Ca(OH) 2 .
(© 2020 International Endodontic Journal. Published by John Wiley & Sons Ltd.)

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