Performance of a UV-A LED system for degradation of aflatoxins B <subscript>1</subscript> and M <subscript>1</subscript> in pure water <subscript>:</subscript> kinetics and cytotoxicity study. - OpacWWW

Tytuł:: Performance of a UV-A LED system for degradation of aflatoxins B 1 and M 1 in pure water : kinetics and cytotoxicity study.
Autorzy:: Stanley J; Food Biosciences and Technology Program, Department of Agricultural and Environmental Sciences, College of Agriculture, Tennessee State University, Nashville, TN, 37209, USA.
Patras A; Food Biosciences and Technology Program, Department of Agricultural and Environmental Sciences, College of Agriculture, Tennessee State University, Nashville, TN, 37209, USA. .
Pendyala B; Food Biosciences and Technology Program, Department of Agricultural and Environmental Sciences, College of Agriculture, Tennessee State University, Nashville, TN, 37209, USA. .
Vergne MJ; Department of Pharmaceutical Sciences, Department of Chemistry and Biochemistry, Lipscomb University, Nashville, TN, 37204, USA.
Bansode RR; Center for Excellence in Post-Harvest Technologies, North Carolina Research Campus, North Carolina Agricultural and Technical State University, Kannapolis, 28081, NC, USA.
Źródło:: Scientific reports [Sci Rep] 2020 Aug 10; Vol. 10 (1), pp. 13473. Date of Electronic Publication: 2020 Aug 10.
Typ publikacji:: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
Język:: English
Imprint Name(s):: Original Publication: London : Nature Publishing Group, copyright 2011-
MeSH Terms:: Aflatoxins/*analysis
Ultraviolet Rays/*adverse effects
Water Purification/*methods
Aflatoxin B1/analysis ; Aflatoxin B1/toxicity ; Aflatoxin M1/analysis ; Aflatoxin M1/toxicity ; Aflatoxins/toxicity ; Chromatography, High Pressure Liquid/methods ; Chromatography, Liquid/methods ; Hep G2 Cells ; Humans ; Kinetics ; Tandem Mass Spectrometry/methods ; Water
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Substance Nomenclature:: 0 (Aflatoxins)
059QF0KO0R (Water)
6795-23-9 (Aflatoxin M1)
9N2N2Y55MH (Aflatoxin B1)
Entry Date(s):: Date Created: 20200812 Date Completed: 20201217 Latest Revision: 20210810
Update Code:: 20240105
PubMed Central ID:: PMC7417570
DOI:: 10.1038/s41598-020-70370-x
PMID:: 32778713
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Pełny tekst

The efficacy of a UV-A light emitting diode system (LED) to reduce the concentrations of aflatoxin B 1 , aflatoxin M 1 (AFB 1 , AFM 1 ) in pure water was studied. This work investigates and reveals the kinetics and main mechanism(s) responsible for the destruction of aflatoxins in pure water and assesses the cytotoxicity in liver hepatocellular cells. Irradiation experiments were conducted using an LED system operating at 365 nm (monochromatic wave-length). Known concentrations of aflatoxins were spiked in water and irradiated at UV-A doses ranging from 0 to 1,200 mJ/cm 2 . The concentration of AFB 1 and AFM 1 was determined by HPLC with fluorescence detection. LC-MS/MS product ion scans were used to identify and semi-quantify degraded products of AFB 1 and AFM 1 . It was observed that UV-A irradiation significantly reduced aflatoxins in pure water. In comparison to control, at dose of 1,200 mJ/cm 2 UV-A irradiation reduced AFB 1 and AFM 1 concentrations by 70 ± 0.27 and 84 ± 1.95%, respectively. We hypothesize that the formation of reactive species initiated by UV-A light may have caused photolysis of AFB 1 and AFM 1 molecules in water. In cell culture studies, our results demonstrated that the increase of UV-A dosage decreased the aflatoxins-induced cytotoxicity in HepG2 cells, and no significant aflatoxin-induced cytotoxicity was observed at UV-A dose of 1,200 mJ/cm 2 . Further results from this study will be used to compare aflatoxins detoxification kinetics and mechanisms involved in liquid foods such as milk and vegetable oils.

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Performance of a UV-A LED system for degradation of aflatoxins B 1 and M 1 in pure water : kinetics and cytotoxicity study.