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Tytuł pozycji:

Mutational spectra of SARS-CoV-2 orf1ab polyprotein and signature mutations in the United States of America.

Tytuł:
Mutational spectra of SARS-CoV-2 orf1ab polyprotein and signature mutations in the United States of America.
Autorzy:
Banerjee S; Environmental Epigenomics Lab, Department of Environmental Science, University of Calcutta, Kolkata, India.; UGC-DAE Consortium for Scientific Research Kolkata Centre, Kolkata, India.
Seal S; Environmental Epigenomics Lab, Department of Environmental Science, University of Calcutta, Kolkata, India.; Department of Zoology, Ramakrishna Mission Vidyamandira, Howrah, India.
Dey R; Environmental Epigenomics Lab, Department of Environmental Science, University of Calcutta, Kolkata, India.; Department of Zoology, Ramakrishna Mission Vidyamandira, Howrah, India.
Mondal KK; Environmental Epigenomics Lab, Department of Environmental Science, University of Calcutta, Kolkata, India.
Bhattacharjee P; Environmental Epigenomics Lab, Department of Environmental Science, University of Calcutta, Kolkata, India.
Źródło:
Journal of medical virology [J Med Virol] 2021 Mar; Vol. 93 (3), pp. 1428-1435. Date of Electronic Publication: 2020 Aug 25.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: New York Ny : Wiley-Liss
Original Publication: New York, Liss.
MeSH Terms:
Mutation*
COVID-19/*virology
SARS-CoV-2/*genetics
Viral Proteins/*genetics
Amino Acid Substitution ; Coronavirus RNA-Dependent RNA Polymerase/chemistry ; Coronavirus RNA-Dependent RNA Polymerase/genetics ; Humans ; Polyproteins/chemistry ; Polyproteins/genetics ; Protein Conformation ; United States ; Viral Nonstructural Proteins/chemistry ; Viral Nonstructural Proteins/genetics ; Viral Proteins/chemistry
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Contributed Indexing:
Keywords: COVID-19; ORF1ab polyprotein; SARS-CoV-2; United States of America; mutational spectra; pandemic
Substance Nomenclature:
0 (ORF1ab polyprotein, SARS-CoV-2)
0 (Polyproteins)
0 (Viral Nonstructural Proteins)
0 (Viral Proteins)
0 (nsp2 protein, SARS-CoV-2)
EC 2.7.7.48 (Coronavirus RNA-Dependent RNA Polymerase)
EC 2.7.7.48 (NSP12 protein, SARS-CoV-2)
Entry Date(s):
Date Created: 20200812 Date Completed: 20210309 Latest Revision: 20220716
Update Code:
20240105
PubMed Central ID:
PMC7436414
DOI:
10.1002/jmv.26417
PMID:
32779784
Czasopismo naukowe
The pandemic COVID-19 outbreak has been caused due to SARS-CoV-2 pathogen, resulting in millions of infections and deaths worldwide, the United States being on top at the present moment. The long, complex orf1ab polyproteins of SARS-CoV-2 play an important role in viral RNA synthesis. To assess the impact of mutations in this important domain, we analyzed 1134 complete protein sequences of the orf1ab polyprotein from the NCBI virus database from affected patients across various states of the United States from December 2019 to 25 April 2020. Multiple sequence alignment using Clustal Omega followed by statistical significance was calculated. Four significant mutations T265I (nsp 2), P4715L (nsp 12), and P5828L and Y5865C (both at nsp 13) were identified in important nonstructural proteins, which function either as replicase or helicase. A comparative analysis shows 265 T→I, 5828 P→L, and 5865Y→C are unique to the United States and not reported from Europe or Asia; while one, 4715 P→L is predominant in both Europe and the United States. Mutational changes in amino acids are predicted to alter the structure and function of the corresponding proteins, thereby, it is imperative to consider the mutational spectra while designing new antiviral therapeutics targeting viral orf1ab.
(© 2020 Wiley Periodicals LLC.)

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