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Tytuł:
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Isolation and phylogenetic analysis of Getah virus from a commercial modified live vaccine against porcine reproductive and respiratory syndrome virus.
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Autorzy:
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Zhou F; College of Animal Veterinary Medicine, Henan Agricultural University, Agricultural Road No. 63, Zhengzhou, Henan, 450002, China.
Wang A; College of Animal Veterinary Medicine, Henan Agricultural University, Agricultural Road No. 63, Zhengzhou, Henan, 450002, China.
Chen L; College of Animal Veterinary Medicine, Henan Agricultural University, Agricultural Road No. 63, Zhengzhou, Henan, 450002, China.
Wang X; College of Animal Veterinary Medicine, Henan Agricultural University, Agricultural Road No. 63, Zhengzhou, Henan, 450002, China.
Cui D; College of Animal Veterinary Medicine, Henan Agricultural University, Agricultural Road No. 63, Zhengzhou, Henan, 450002, China.
Chang H; College of Animal Veterinary Medicine, Henan Agricultural University, Agricultural Road No. 63, Zhengzhou, Henan, 450002, China. Electronic address: .
Wang C; College of Animal Veterinary Medicine, Henan Agricultural University, Agricultural Road No. 63, Zhengzhou, Henan, 450002, China. Electronic address: .
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Źródło:
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Molecular and cellular probes [Mol Cell Probes] 2020 Oct; Vol. 53, pp. 101650. Date of Electronic Publication: 2020 Aug 08.
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Original Publication: London ; New York : Academic Press, c1987-
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MeSH Terms:
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Alphavirus/*classification
Porcine Reproductive and Respiratory Syndrome/*prevention & control
Viral Vaccines/*analysis
Alphavirus/genetics ; Alphavirus/isolation & purification ; Animals ; Cell Line ; China ; Drug Contamination ; Horses ; Japan ; Phylogeny ; Phylogeography ; Swine
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Contributed Indexing:
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Keywords: Getah virus; Isolation; Sequence analysis; Vaccine
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Substance Nomenclature:
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0 (Viral Vaccines)
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Entry Date(s):
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Date Created: 20200812 Date Completed: 20210713 Latest Revision: 20210713
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Update Code:
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20240105
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DOI:
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10.1016/j.mcp.2020.101650
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PMID:
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32781023
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In the present study, Getah virus (GETV) isolate, GETV-V1, was isolated from a commercial PRRSV attenuated live vaccine (MLV), which has been widely used to immunize pigs against porcine reproductive and respiratory syndrome virus (PRRSV). Further analysis demonstrated that nine batches of the PRRSV MLV vaccine (three batches per year from 2017 to 2019) from the same manufacturer were all positive for GETV. Genomic analyses indicated that the GETV-V1 isolate shared the highest sequence identity with the GETV strain, 16-I-674, which was isolated from horses in Japan. The phylogenetic analysis based on the genomic sequences showed that the GETV-V1 strain was clustered with the Japanese GETV strains. Taken together, this is the first report of GETV contamination in live swine vaccines in China. Our findings demonstrate that immunization with commercial live vaccines might be a potential novel route of GETV transmission in swine. This highlights the need for more extensive monitoring of commercial live vaccines.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
