Clinical features of irreversible rejection after allogeneic uterus transplantation in cynomolgus macaques.

Tytuł:: Clinical features of irreversible rejection after allogeneic uterus transplantation in cynomolgus macaques.
Autorzy:: Kisu I; Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi , Shinjuku-ku, Tokyo, 1608582, Japan. .
Emoto K; Department of Pathology, Keio University School of Medicine, Shinjuku, Tokyo, 1608582, Japan.
Masugi Y; Department of Pathology, Keio University School of Medicine, Shinjuku, Tokyo, 1608582, Japan.
Yamada Y; Department of Pediatric Surgery, Keio University School of Medicine, Shinjuku, Tokyo, 1608582, Japan.
Matsubara K; Department of Surgery, Keio University School of Medicine, Shinjuku, Tokyo, 1608582, Japan.
Obara H; Department of Surgery, Keio University School of Medicine, Shinjuku, Tokyo, 1608582, Japan.
Matoba Y; Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi , Shinjuku-ku, Tokyo, 1608582, Japan.
Banno K; Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi , Shinjuku-ku, Tokyo, 1608582, Japan.
Kato Y; Department of Surgery, Division of Gastroenterological and General Surgery, School of Medicine, Showa University, Shinagawa, Tokyo, 1428666, Japan.
Saiki Y; Department of Anesthesiology, Saiseikai Kanagawaken Hospital, Yokohama, Kanagawa, 2210821, Japan.
Itagaki I; Research Center for Animal Life Science, Shiga University of Medical Science, Ōtsu, Shiga, 5202192, Japan.; The Corporation for Production and Research of Laboratory Primates, Tsukuba, Ibaraki, 3050003, Japan.
Kawamoto I; Research Center for Animal Life Science, Shiga University of Medical Science, Ōtsu, Shiga, 5202192, Japan.
Iwatani C; Research Center for Animal Life Science, Shiga University of Medical Science, Ōtsu, Shiga, 5202192, Japan.
Murase M; Research Center for Animal Life Science, Shiga University of Medical Science, Ōtsu, Shiga, 5202192, Japan.
Nakagawa T; Research Center for Animal Life Science, Shiga University of Medical Science, Ōtsu, Shiga, 5202192, Japan.
Tsuchiya H; Research Center for Animal Life Science, Shiga University of Medical Science, Ōtsu, Shiga, 5202192, Japan.
Ishigaki H; Department of Pathology, Shiga University of Medical Science, Ōtsu, Shiga, 5202192, Japan.
Urano H; Safety Research Center, Ina Research Inc., Ina, Nagano, 3994501, Japan.
Ema M; Research Center for Animal Life Science, Shiga University of Medical Science, Ōtsu, Shiga, 5202192, Japan.
Ogasawara K; Research Center for Animal Life Science, Shiga University of Medical Science, Ōtsu, Shiga, 5202192, Japan.; Department of Pathology, Shiga University of Medical Science, Ōtsu, Shiga, 5202192, Japan.
Aoki D; Department of Obstetrics and Gynecology, Keio University School of Medicine, 35 Shinanomachi , Shinjuku-ku, Tokyo, 1608582, Japan.
Nakagawa K; Safety Research Center, Ina Research Inc., Ina, Nagano, 3994501, Japan.
Shiina T; Department of Molecular Life Science, Division of Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Hiratsuka, Kanagawa, 2591193, Japan.
Źródło:: Scientific reports [Sci Rep] 2020 Aug 17; Vol. 10 (1), pp. 13910. Date of Electronic Publication: 2020 Aug 17.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: London : Nature Publishing Group, copyright 2011-
MeSH Terms:: Graft Rejection/*pathology
Uterus/*transplantation
Animals ; C-Reactive Protein/metabolism ; Female ; Graft Rejection/blood ; Immunosuppression Therapy ; Indocyanine Green/chemistry ; L-Lactate Dehydrogenase/metabolism ; Laparotomy ; Leukocyte Count ; Macaca fascicularis ; Optical Imaging ; Time Factors ; Transplantation, Homologous ; Ultrasonography ; Uterus/diagnostic imaging ; Uterus/pathology
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Substance Nomenclature:: 9007-41-4 (C-Reactive Protein)
EC 1.1.1.27 (L-Lactate Dehydrogenase)
IX6J1063HV (Indocyanine Green)
Entry Date(s):: Date Created: 20200819 Date Completed: 20201210 Latest Revision: 20211204
Update Code:: 20240104
PubMed Central ID:: PMC7431528
DOI:: 10.1038/s41598-020-70914-1
PMID:: 32807830
: Czasopismo naukowe

Pełny tekst

Uterus transplantation (UTx) is a potential option for women with uterine factor infertility to have a child. The clinical features indicating irreversible rejection of the uterus are unknown. In our experimental series of allogeneic UTx in cynomolgus macaques, six female macaques were retrospectively examined, which were unresponsive to treatment with immunosuppressants (i.e. irreversible rejection). Clinical features including general condition, hematology, uterine size, indocyanine green (ICG) fluorescence imaging by laparotomy, and histopathological findings of the removed uterus were evaluated. In all cases, general condition was good at the time of diagnosis of irreversible rejection and thereafter. Laboratory evaluation showed temporary increases in white blood cells, lactate dehydrogenase and C-reactive protein, then these levels tended to decrease gradually. In transabdominal ultrasonography, the uterus showed time-dependent shrinkage after transient swelling at the time of diagnosis of irreversible rejection. In laparotomy, a whitish transplanted uterus was observed and enhancement of the transplanted uterus was absent in ICG fluorescence imaging. Histopathological findings in each removed uterus showed hyalinized fibrosis, endometrial deficit, lymphocytic infiltration and vasculitis. These findings suggest that uterine transplantation rejection is not fatal, in contrast to rejection of life-supporting organs. Since the transplanted uterus with irreversible rejection atrophies naturally, hysterectomy may be unnecessary.

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