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Tytuł pozycji:

T cell and chemokine receptors differentially control CD8 T cell motility behavior in the infected airways immediately before and after virus clearance in a primary infection.

Tytuł :
T cell and chemokine receptors differentially control CD8 T cell motility behavior in the infected airways immediately before and after virus clearance in a primary infection.
Autorzy :
Emo K; David H. Smith Center for Vaccine Biology and Immunology, Department of Microbiology and Immunology, Rochester, NY, United States of America.
Reilly EC; David H. Smith Center for Vaccine Biology and Immunology, Department of Microbiology and Immunology, Rochester, NY, United States of America.
Sportiello M; David H. Smith Center for Vaccine Biology and Immunology, Department of Microbiology and Immunology, Rochester, NY, United States of America.
Yang H; Department of Biostatistics and Computational Biology, University of Rochester Medical Center, Rochester, NY, United States of America.
Topham DJ; David H. Smith Center for Vaccine Biology and Immunology, Department of Microbiology and Immunology, Rochester, NY, United States of America.
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Źródło :
PloS one [PLoS One] 2020 Aug 20; Vol. 15 (8), pp. e0227157. Date of Electronic Publication: 2020 Aug 20 (Print Publication: 2020).
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
Język :
English
Imprint Name(s) :
Original Publication: San Francisco, CA : Public Library of Science
MeSH Terms :
CD8-Positive T-Lymphocytes/*immunology
Cell Movement/*physiology
Influenza A virus/*immunology
Animals ; CD8-Positive T-Lymphocytes/metabolism ; CD8-Positive T-Lymphocytes/physiology ; Cell Movement/drug effects ; Chemokines/immunology ; Influenza A virus/pathogenicity ; Male ; Mice ; Mice, Inbred C57BL ; Orthomyxoviridae ; Orthomyxoviridae Infections/immunology ; Pertussis Toxin/metabolism ; Pertussis Toxin/pharmacology ; Receptors, Chemokine ; Receptors, G-Protein-Coupled/metabolism
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Grant Information :
P01 AI102851 United States AI NIAID NIH HHS; T32 GM007356 United States GM NIGMS NIH HHS
Substance Nomenclature :
0 (Chemokines)
0 (Receptors, Chemokine)
0 (Receptors, G-Protein-Coupled)
EC 2.4.2.31 (Pertussis Toxin)
Entry Date(s) :
Date Created: 20200821 Date Completed: 20200928 Latest Revision: 20200928
Update Code :
20201023
PubMed Central ID :
PMC7444504
DOI :
10.1371/journal.pone.0227157
PMID :
32817719
Czasopismo naukowe
In mice, experimental influenza virus infection stimulates CD8 T cell infiltration of the airways. Virus is cleared by day 9, and between days 8 and 9 there is an abrupt change in CD8 T cell motility behavior transitioning from low velocity and high confinement on day 8, to high velocity with continued high confinement on day 9. We hypothesized that loss of virus and/or antigen signals in the context of high chemokine levels drives the T cells into a rapid surveillance mode. Virus infection induces chemokine production, which may change when the virus is cleared. We therefore sought to examine this period of rapid changes to the T cell environment in the tissue and seek evidence on the roles of peptide-MHC and chemokine receptor interactions. Experiments were performed to block G protein coupled receptor (GPCR) signaling with Pertussis toxin (Ptx). Ptx treatment generally reduced cell velocities and mildly increased confinement suggesting chemokine mediated arrest (velocity <2 μm/min) (Friedman RS, 2005), except on day 8 when velocity increased and confinement was relieved. Blocking specific peptide-MHC with monoclonal antibody unexpectedly decreased velocities on days 7 through 9, suggesting TCR/peptide-MHC interactions promote cell mobility in the tissue. Together, these results suggest the T cells are engaged with antigen bearing and chemokine producing cells that affect motility in ways that vary with the day after infection. The increase in velocities on day 9 were reversed by addition of specific peptide, consistent with the idea that antigen signals become limiting on day 9 compared to earlier time points. Thus, antigen and chemokine signals act to alternately promote and restrict CD8 T cell motility until the point of virus clearance, suggesting the switch in motility behavior on day 9 may be due to a combination of limiting antigen in the presence of high chemokine signals as the virus is cleared.
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