Phosphodiester modifications in mRNA poly(A) tail prevent deadenylation without compromising protein expression.

Szczegóły
Abstrakt

Tytuł:: Phosphodiester modifications in mRNA poly(A) tail prevent deadenylation without compromising protein expression.
Autorzy:: Strzelecka D; Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, 02-093 Warsaw, Poland.
Smietanski M; Centre of New Technologies, University of Warsaw, 02-097 Warsaw, Poland.
Sikorski PJ; Centre of New Technologies, University of Warsaw, 02-097 Warsaw, Poland.
Warminski M; Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, 02-093 Warsaw, Poland.
Kowalska J; Division of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, 02-093 Warsaw, Poland.
Jemielity J; Centre of New Technologies, University of Warsaw, 02-097 Warsaw, Poland.
Źródło:: RNA (New York, N.Y.) [RNA] 2020 Dec; Vol. 26 (12), pp. 1815-1837. Date of Electronic Publication: 2020 Aug 20.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Publication: <2003->: Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press
Original Publication: New York, NY : Cambridge University Press, c1995-
MeSH Terms:: Protein Biosynthesis*
DNA-Directed RNA Polymerases/*metabolism
Phosphorothioate Oligonucleotides/*chemistry
Poly A/*metabolism
RNA, Messenger/*metabolism
Adenosine Triphosphate/metabolism ; Animals ; DNA-Directed RNA Polymerases/genetics ; Dendritic Cells/cytology ; Dendritic Cells/metabolism ; HeLa Cells ; Humans ; Mice ; Poly A/chemistry ; Poly A/genetics ; Protein Processing, Post-Translational ; RNA, Messenger/chemistry ; RNA, Messenger/genetics ; Transcription, Genetic
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Contributed Indexing:: Keywords: deadenylation; mRNA modification; resistance; transcription; translation
Substance Nomenclature:: 0 (Phosphorothioate Oligonucleotides)
0 (RNA, Messenger)
24937-83-5 (Poly A)
8L70Q75FXE (Adenosine Triphosphate)
EC 2.7.7.6 (DNA-Directed RNA Polymerases)
Entry Date(s):: Date Created: 20200822 Date Completed: 20210125 Latest Revision: 20240330
Update Code:: 20240330
PubMed Central ID:: PMC7668260
DOI:: 10.1261/rna.077099.120
PMID:: 32820035
: Czasopismo naukowe

Chemical modifications enable preparation of mRNAs with augmented stability and translational activity. In this study, we explored how chemical modifications of 5',3'-phosphodiester bonds in the mRNA body and poly(A) tail influence the biological properties of eukaryotic mRNA. To obtain modified and unmodified in vitro transcribed mRNAs, we used ATP and ATP analogs modified at the α-phosphate (containing either O-to-S or O-to-BH 3 substitutions) and three different RNA polymerases-SP6, T7, and poly(A) polymerase. To verify the efficiency of incorporation of ATP analogs in the presence of ATP, we developed a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantitative assessment of modification frequency based on exhaustive degradation of the transcripts to 5'-mononucleotides. The method also estimated the average poly(A) tail lengths, thereby providing a versatile tool for establishing a structure-biological property relationship for mRNA. We found that mRNAs containing phosphorothioate groups within the poly(A) tail were substantially less susceptible to degradation by 3'-deadenylase than unmodified mRNA and were efficiently expressed in cultured cells, which makes them useful research tools and potential candidates for future development of mRNA-based therapeutics.
(© 2020 Strzelecka et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society.)

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