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Tytuł pozycji:

A selective and highly sensitive high performance liquid chromatography with fluorescence derivatization approach based on Sonogashira coupling reaction for determination of ethinyl estradiol in river water samples.

Tytuł :
A selective and highly sensitive high performance liquid chromatography with fluorescence derivatization approach based on Sonogashira coupling reaction for determination of ethinyl estradiol in river water samples.
Autorzy :
Ali MFB; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, 71526, Assiut, Egypt; Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
Uejo Y; Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
Kishikawa N; Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan. Electronic address: .
Ohyama K; Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
Kuroda N; Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki 852-8521, Japan.
Pokaż więcej
Źródło :
Journal of chromatography. A [J Chromatogr A] 2020 Sep 27; Vol. 1628, pp. 461440. Date of Electronic Publication: 2020 Jul 29.
Typ publikacji :
Journal Article
Język :
English
Imprint Name(s) :
Original Publication: Amsterdam ; New York : Elsevier, 1993-
MeSH Terms :
Chromatography, High Pressure Liquid*
Spectrometry, Fluorescence*
Environmental Monitoring/*methods
Ethinyl Estradiol/*analysis
Rivers/*chemistry
Endocrine Disruptors/analysis ; Fluorescence ; Solid Phase Extraction ; Water Pollutants, Chemical/analysis
Contributed Indexing :
Keywords: Ethinyl estradiol; Fluorescence derivatization; HPLC analysis; Sonogashira coupling; Water analysis
Substance Nomenclature :
0 (Endocrine Disruptors)
0 (Water Pollutants, Chemical)
423D2T571U (Ethinyl Estradiol)
Entry Date(s) :
Date Created: 20200822 Date Completed: 20201016 Latest Revision: 20201016
Update Code :
20201023
DOI :
10.1016/j.chroma.2020.461440
PMID :
32822980
Czasopismo naukowe
A selective and highly sensitive high performance liquid chromatography (HPLC) with fluorescence derivatization method was developed for determination of ethinyl estradiol (EE); one of endocrine-disrupting compounds (EDCs). The fluorescence derivatization procedure was based on Sonogashira coupling reaction using 4-(4, 5-diphenyl-1H-imidazole-2-yl) iodobenzene (DIB-I), a fluorescence labeling reagent, to derivatize EE in presence of copper and palladium ions. The formed fluorescent product was separated on Cosmosil 5C 18 MS-II by an isocratic elution with a mobile phase composed of acetonitrile: 5.0 mM Tris-HNO 3 buffer, pH 7.4 (60:40, v/v %). The detection wavelengths were set at 310 and 400 nm as excitation and emission wavelengths, respectively. Various parameters affecting derivatization reaction were optimized. Further, the proposed method was validated and a good linearity with low detection limit (S/N=3) 7.4 ng L -1 was obtained in water sample after a simple solid-phase disk extraction (C 18 SPE disk) method. The proposed method was successfully applied for detection of EE in river water samples in order to monitor EE concentration and to distinguish its effect on the ecosystem and human health.
(Copyright © 2020. Published by Elsevier B.V.)

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