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Tytuł:
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Protein Determination with Molecularly Imprinted Polymer Recognition Combined with Birefringence Liquid Crystal Detection.
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Autorzy:
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Cieplak M; Institute of Physical Chemistry Polish Academy of Sciences, Kasprzaka Str. 44/52, 01-224 Warsaw, Poland.
Węgłowski R; Faculty of Advanced Technologies and Chemistry, Military University of Technology, Kaliskiego Str. 2, 00-908 Warsaw, Poland.
Iskierko Z; Institute of Physical Chemistry Polish Academy of Sciences, Kasprzaka Str. 44/52, 01-224 Warsaw, Poland.
Węgłowska D; Faculty of Advanced Technologies and Chemistry, Military University of Technology, Kaliskiego Str. 2, 00-908 Warsaw, Poland.
Sharma PS; Institute of Physical Chemistry Polish Academy of Sciences, Kasprzaka Str. 44/52, 01-224 Warsaw, Poland.
Noworyta KR; Institute of Physical Chemistry Polish Academy of Sciences, Kasprzaka Str. 44/52, 01-224 Warsaw, Poland.
D'Souza F; Department of Chemistry, University of North Texas, 1155 Union Circle No 305070, Denton, TX 76203-5017, USA.
Kutner W; Institute of Physical Chemistry Polish Academy of Sciences, Kasprzaka Str. 44/52, 01-224 Warsaw, Poland.; Faculty of Mathematics and Natural Sciences, School of Sciences, Cardinal Stefan Wyszynski University in Warsaw, Wóycickiego Str. 1/3, 01-815 Warsaw, Poland.
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Źródło:
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Sensors (Basel, Switzerland) [Sensors (Basel)] 2020 Aug 20; Vol. 20 (17). Date of Electronic Publication: 2020 Aug 20.
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Typ publikacji:
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Letter
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Język:
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English
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Imprint Name(s):
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Original Publication: Basel, Switzerland : MDPI, c2000-
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MeSH Terms:
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Liquid Crystals*
Molecularly Imprinted Polymers*
Proteins/*analysis
Birefringence ; Humans ; Molecular Imprinting ; Serum Albumin, Human
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References:
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Grant Information:
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NCN 2015/19/B/ST4/03473 National Science Center of Poland; No 079/STYP/13/2018 Polish Ministry of Science and Education; UGB 22-764 Military University of Technology funds for the year 2020
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Contributed Indexing:
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Keywords: human serum albumin; liquid crystal; molecularly imprinted polymer
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Substance Nomenclature:
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0 (Molecularly Imprinted Polymers)
0 (Proteins)
ZIF514RVZR (Serum Albumin, Human)
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Entry Date(s):
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Date Created: 20200823 Date Completed: 20210326 Latest Revision: 20210326
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Update Code:
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20240105
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PubMed Central ID:
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PMC7547379
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DOI:
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10.3390/s20174692
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PMID:
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32825278
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Liquid crystal-based sensors offer the advantage of high sensitivity at a low cost. However, they often lack selectivity altogether or require costly and unstable biomaterials to impart this selectivity. To incur this selectivity, we herein integrated a molecularly imprinted polymer (MIP) film recognition unit with a liquid crystal (LC) in an optical cell transducer. We tested the resulting chemosensor for protein determination. We examined two different LCs, each with a different optical birefringence. That way, we revealed the influence of that parameter on the sensitivity of the (human serum albumin)-templated (MIP-HSA) LC chemosensor. The response of this chemosensor with the (MIP-HSA)-recognizing film was linear from 2.2 to 15.2 µM HSA, with a limit of detection of 2.2 µM. These values are sufficient to use the devised chemosensor for HSA determination in biological samples. Importantly, the imprinting factor ( IF ) of this chemosensor was appreciable, reaching IF = 3.7. This IF value indicated the predominant binding of the HSA through specific rather than nonspecific interactions with the MIP.
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