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Tytuł pozycji:

Systematic analysis of different pluripotent stem cell-derived cardiac myocytes as potential testing model for cardiocytoprotection.

Tytuł:
Systematic analysis of different pluripotent stem cell-derived cardiac myocytes as potential testing model for cardiocytoprotection.
Autorzy:
Pálóczi J; Cardiovascular Research Group, Department of Biochemistry, University of Szeged, 6720 Hungary.
Szántai Á; Cardiovascular Research Group, Department of Biochemistry, University of Szeged, 6720 Hungary.
Kobolák J; Biotalentum Ltd., Gödöllő, 2100 Hungary.
Bock I; Biotalentum Ltd., Gödöllő, 2100 Hungary.
Ruivo E; Cardiovascular Research Group, Department of Biochemistry, University of Szeged, 6720 Hungary.
Kiss B; Cardiovascular Research Group, Department of Biochemistry, University of Szeged, 6720 Hungary; MTA-SE System Pharmacology Research Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, 1085 Hungary.
Gáspár R; Cardiovascular Research Group, Department of Biochemistry, University of Szeged, 6720 Hungary.
Pipis J; Pharmahungary Group, Szeged, 6722 Hungary.
Ocsovszki I; Cardiovascular Research Group, Department of Biochemistry, University of Szeged, 6720 Hungary.
Táncos Z; Biotalentum Ltd., Gödöllő, 2100 Hungary.
Fehér A; Biotalentum Ltd., Gödöllő, 2100 Hungary.
Dinnyés A; Biotalentum Ltd., Gödöllő, 2100 Hungary; Molecular Animal Biotechnology Laboratory, Szent István University, Gödöllő, 2100 Hungary.
Onódi Z; MTA-SE System Pharmacology Research Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, 1085 Hungary.
Madonna R; Institute of Cardiology, Department of Surgical, Medical and Molecular Pathology and Critical Area Medicine, University of Pisa, 56124 Pisa; Internal Medicine, Cardiology Division, University of Texas Medical School in Houston, Houston, Texas.
Ferdinandy P; Cardiovascular Research Group, Department of Biochemistry, University of Szeged, 6720 Hungary; Pharmahungary Group, Szeged, 6722 Hungary; MTA-SE System Pharmacology Research Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, 1085 Hungary; Cardiovascular Research Group, Department of Pharmacology and Pharmacotherapy, University of Szeged, 6720, Hungary.
Görbe A; Cardiovascular Research Group, Department of Biochemistry, University of Szeged, 6720 Hungary; Pharmahungary Group, Szeged, 6722 Hungary; MTA-SE System Pharmacology Research Group, Department of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest, 1085 Hungary; Cardiovascular Research Group, Department of Pharmacology and Pharmacotherapy, University of Szeged, 6720, Hungary. Electronic address: .
Źródło:
Vascular pharmacology [Vascul Pharmacol] 2020 Oct - Nov; Vol. 133-134, pp. 106781. Date of Electronic Publication: 2020 Aug 19.
Typ publikacji:
Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Original Publication: New York, NY : Elsevier Science, c2002-
MeSH Terms:
Cell Differentiation*
Myocardial Reperfusion Injury/*prevention & control
Myocytes, Cardiac/*drug effects
Nitric Oxide Donors/*pharmacology
Pluripotent Stem Cells/*drug effects
S-Nitroso-N-Acetylpenicillamine/*pharmacology
Cell Line ; Cell Survival/drug effects ; Humans ; Myocardial Reperfusion Injury/metabolism ; Myocardial Reperfusion Injury/pathology ; Myocytes, Cardiac/metabolism ; Myocytes, Cardiac/pathology ; Oxidative Stress/drug effects ; Phenotype ; Pluripotent Stem Cells/metabolism ; Pluripotent Stem Cells/pathology ; Troponin I/metabolism ; Vascular Cell Adhesion Molecule-1/metabolism
Contributed Indexing:
Keywords: Cardiocytoprotection; Cell culture; Drug screening; Embryoid body; In vitro; Induced pluripotent stem cell
Substance Nomenclature:
0 (Nitric Oxide Donors)
0 (Troponin I)
0 (Vascular Cell Adhesion Molecule-1)
79032-48-7 (S-Nitroso-N-Acetylpenicillamine)
Entry Date(s):
Date Created: 20200823 Date Completed: 20201021 Latest Revision: 20201021
Update Code:
20240105
DOI:
10.1016/j.vph.2020.106781
PMID:
32827678
Czasopismo naukowe
Introduction: Stem cell-derived cardiac myocytes are potential sources for testing cardiocytoprotective molecules against ischemia/reperfusion injury in vitro.
Materials and Methods: Here we performed a systematic analysis of two different induced pluripotent stem cell lines (iPSC 3.4 and 4.1) and an embryonic stem cell (ESC) line-derived cardiac myocytes at two different developmental stages. Cell viability in simulated ischemia/reperfusion (SI/R)-induced injury and a known cardiocytoprotective NO-donor, S-nitroso-n-acetylpenicillamine (SNAP) was tested.
Results: After analysis of full embryoid bodies (EBs) and cardiac marker (VCAM and cardiac troponin I) positive cells of three lines at 6 conditions (32 different conditions altogether), we found significant SI/R injury-induced cell death in both full EBs and VCAM+ cardiac cells at later stage of their differentiation. Moreover, full EBs of the iPS 4.1 cell line after oxidative stress induction by SNAP was protected at day-8 samples.
Conclusion: We have shown that 4.1 iPS-derived cardiomyocyte line could serve as a testing platform for cardiocytoprotection.
Competing Interests: Declaration of Competing Interest Author Kobolák, Julianna; Bock, István; Fehér, Anita; Dinnyes, Andras was employed by the company Biotalentum Ltd. Author Anikó Görbe, Péter Ferdinandy and Judit Pipis was employed by the company Pharmahungary Group Ltd. The remaining authors declare that the research was conducted in the absence of any commercial for financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)

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