Differently Expression Analysis and Function Prediction of Long Non-coding RNAs in Duck Embryo Fibroblast Cells Infected by Duck Tembusu Virus.

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Tytuł:: Differently Expression Analysis and Function Prediction of Long Non-coding RNAs in Duck Embryo Fibroblast Cells Infected by Duck Tembusu Virus.
Autorzy:: Lin Y; College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an, China.; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai'an, China.
Yang J; College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an, China.; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai'an, China.
He D; College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an, China.; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai'an, China.
Li X; College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an, China.; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai'an, China.
Li J; College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an, China.; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai'an, China.
Tang Y; College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an, China.; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai'an, China.
Diao Y; College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an, China.; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai'an, China.
Źródło:: Frontiers in immunology [Front Immunol] 2020 Aug 04; Vol. 11, pp. 1729. Date of Electronic Publication: 2020 Aug 04 (Print Publication: 2020).
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: [Lausanne : Frontiers Research Foundation]
MeSH Terms:: Transcriptome*
Ducks/*virology
Fibroblasts/*virology
Flavivirus/*pathogenicity
Flavivirus Infections/*veterinary
Poultry Diseases/*virology
RNA, Long Noncoding/*genetics
Animals ; Cells, Cultured ; Ducks/embryology ; Ducks/genetics ; Ducks/immunology ; Fibroblasts/immunology ; Fibroblasts/metabolism ; Flavivirus/immunology ; Flavivirus Infections/genetics ; Flavivirus Infections/immunology ; Flavivirus Infections/virology ; Gene Expression Profiling/veterinary ; Gene Regulatory Networks ; High-Throughput Nucleotide Sequencing/veterinary ; Host-Pathogen Interactions ; Poultry Diseases/genetics ; Poultry Diseases/immunology ; RNA, Long Noncoding/metabolism ; RNA-Seq/veterinary ; Real-Time Polymerase Chain Reaction/veterinary
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Contributed Indexing:: Keywords: DEFs; DTMUV infection; RNA-Seq; gene regulation; lncRNA expression
Substance Nomenclature:: 0 (RNA, Long Noncoding)
SCR Organism:: Tembusu virus
Entry Date(s):: Date Created: 20200828 Date Completed: 20210412 Latest Revision: 20210412
Update Code:: 20240105
PubMed Central ID:: PMC7417515
DOI:: 10.3389/fimmu.2020.01729
PMID:: 32849615
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Duck Tembusu virus (DTMUV), the causative agent of egg-drop syndrome, has caused substantial economic losses to duck industry. DTMUV infection leads to profound changes of host cells, including transcriptome and proteome. However, the lncRNA expression profile and the biological function of lncRNA have not been revealed. Therefore, DTMUV was used to inoculate duck embryo fibroblast cells (DEFs) for high-throughput RNA-sequencing (RNA-Seq). The results showed that 34 and 339 differently expressed lncRNAs were, respectively, identified at 12 and 24 h post-infection (hpi). To analyze their biological functions, target genes in cis were searched and the regulatory network was formed. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that the target genes were strongly associated with immune system, signaling molecular and interaction, endocrine system, and signal transduction. The differently expressed lncRNAs were selected and verified by quantitative real-time polymerase chain reaction (RT-qPCR). Our study, for the first time, analyzed a comprehensive lncRNA expression profile in DEFs following DTMUV infection. The analysis provided a view on the important roles of lncRNAs in gene regulation and DTMUV infection.
(Copyright © 2020 Lin, Yang, He, Li, Li, Tang and Diao.)

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