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Tytuł pozycji:

Fluorescence In Situ Hybridization (FISH) Detection of Chromosomal 12p Anomalies in Testicular Germ Cell Tumors.

Tytuł:
Fluorescence In Situ Hybridization (FISH) Detection of Chromosomal 12p Anomalies in Testicular Germ Cell Tumors.
Autorzy:
Cheng L; Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN, USA. liang_.; Department of Urology, Indiana University School of Medicine, Indianapolis, IN, USA. liang_.
Davidson DD; Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN, USA.
Montironi R; Institute of Pathological Anatomy and Histopathology, School of Medicine, Polytechnic University of the Marche Region (Ancona), United Hospitals, Ancona, Italy.
Wang M; Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN, USA.
Lopez-Beltran A; Department of Pathology and Surgery, Faculty of Medicine, University of Cordoba, Cordoba, Spain.
Masterson TA; Department of Urology, Indiana University School of Medicine, Indianapolis, IN, USA.
Albany C; Department of Medicine, Division of Hematology and Oncology, Indiana University Simon Cancer Center, Indianapolis, IN, USA.
Zhang S; Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, Indianapolis, IN, USA.
Źródło:
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2195, pp. 49-63.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,
MeSH Terms:
Chromosome Aberrations*
Chromosomes, Human, Pair 12*
In Situ Hybridization, Fluorescence*/methods
In Situ Hybridization, Fluorescence*/standards
Neoplasms, Germ Cell and Embryonal/*diagnosis
Neoplasms, Germ Cell and Embryonal/*genetics
Testicular Neoplasms/*diagnosis
Testicular Neoplasms/*genetics
Biomarkers, Tumor ; Diagnosis, Differential ; Genetic Association Studies ; Genetic Predisposition to Disease ; Humans ; Immunohistochemistry ; Male ; Quality Control
References:
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Fitzmaurice C, Allen C, Barber RM, Barregard L, Bhutta ZA, Brenner H et al (2017) Global, regional, and national cancer incidence, mortality, years of life lost, years lived with disability, and disability-adjusted life-years for 32 cancer groups, 1990 to 2015: a systematic analysis for the global burden of disease study. JAMA Oncol 3:524–548. (PMID: 10.1001/jamaoncol.2017.1747)
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Suijkerbuijk RF, Looijenga L, de Jong B, Oosterhuis JW, Cassiman JJ, Geurts van Kessel A (1992) Verification of isochromosome 12p and identification of other chromosome 12 aberrations in gonadal and extragonadal human germ cell tumors by bicolor double fluorescence in situ hybridization. Cancer Genet Cytogenet 63:8–16. (PMID: 10.1016/0165-4608(92)90056-E)
Mostert MM, van de Pol M, van Echten J, Olde Weghuis D, Geurts van Kessel A, Oosterhuis JW et al (1996) Fluorescence in situ hybridization-based approaches for detection of 12p overrepresentation, in particular i(12p), in cell lines of human testicular germ cell tumors of adults. Cancer Genet Cytogenet 87:95–102. (PMID: 10.1016/0165-4608(95)00233-2)
Cheng L, Zhang S, Eble JN, Beck SD, Foster RS, Wang M et al (2012) Molecular genetic evidence supporting the neoplastic nature of fibrous stroma in testicular teratoma. Mod Pathol 25:1432–1438. (PMID: 10.1038/modpathol.2012.99)
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Contributed Indexing:
Keywords: Differential diagnosis; Fluorescence in situ hybridization (FISH); Germ cell tumor; Isochromosome 12p/chromosomal 12p anomalies; Testis
Substance Nomenclature:
0 (Biomarkers, Tumor)
SCR Disease Name:
Testicular Germ Cell Tumor
Entry Date(s):
Date Created: 20200828 Date Completed: 20210324 Latest Revision: 20210828
Update Code:
20240105
DOI:
10.1007/978-1-0716-0860-9_4
PMID:
32852756
Czasopismo naukowe
Gains of genetic material or internal rearrangements of chromosome 12p, including 12p overrepresentation or isochromosome 12p [i(12p)], are observed in virtually all germ cell tumors (GCT), in all histologic subtypes, and from various body locations. The chromosomal region involved in these alterations contains the growth and survival promoting oncogene KRAS (12p12.1). Gains or rearrangements of 12p characterize GCT from in situ to chemoresistant stages. Fluorescence in situ hybridization (FISH) detection of chromosome 12p anomalies is a sensitive and specific test for the diagnosis of germ cell tumors. Here we provide a detailed protocol for FISH detection of isochromosome 12p and chromosome 12p overrepresentation. The method is helpful for diagnosis of germ cell origin, and for selection of patients who may benefit from cisplatin-based chemotherapy.

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