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Tytuł:
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Assessment of In Vitro Anti-melanoma Potential of Ephedranthus pisocarpus R.E.Fr.
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Autorzy:
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Santos LKB; Medicinal Plants Research Center, Federal University of Piauí, Teresina, Brazil.
Veras MDA; Department of Chemistry, Federal University of Piauí, Teresina, Brazil.
Marques KKG; Department of Pharmaceutical Sciences, Federal University of Paraíba, João Pessoa, Brazil.
DE Moraes Alves MM; Medicinal Plants Research Center, Federal University of Piauí, Teresina, Brazil.; Department of Veterinary Morphophysiology, Federal University of Piauí, Teresina, Brazil.
Mendes AN; Department of Biophysics and Physiology, Federal University of Piauí, Teresina, Brazil.
DE Amorim Carvalho FA; Medicinal Plants Research Center, Federal University of Piauí, Teresina, Brazil.
Sobral MV; Department of Pharmaceutical Sciences, Federal University of Paraíba, João Pessoa, Brazil.
Chaves MH; Department of Chemistry, Federal University of Piauí, Teresina, Brazil.
GonÇalves JCR; Medicinal Plants Research Center, Federal University of Piauí, Teresina, Brazil .; Department of Pharmaceutical Sciences, Federal University of Paraíba, João Pessoa, Brazil.
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Źródło:
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Anticancer research [Anticancer Res] 2020 Sep; Vol. 40 (9), pp. 5015-5024.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Attiki, Greece : International Institute of Anticancer Research
Original Publication: Athens, Greece : Potamitis Press
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MeSH Terms:
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Annonaceae/*chemistry
Antineoplastic Agents, Phytogenic/*pharmacology
Plant Extracts/*pharmacology
Animals ; Antineoplastic Agents, Phytogenic/chemistry ; Antineoplastic Agents, Phytogenic/isolation & purification ; Apoptosis/drug effects ; Brazil ; Cell Line, Tumor ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; Cytotoxicity, Immunologic ; Dose-Response Relationship, Drug ; Hemolysis ; Humans ; Melanoma, Experimental ; Mice ; Molecular Structure ; Plant Extracts/chemistry ; Plant Extracts/isolation & purification
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Contributed Indexing:
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Keywords: E. pisocarpus; Melanoma; apoptosis; natural products; photoprotection; spathulenol
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Substance Nomenclature:
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0 (Antineoplastic Agents, Phytogenic)
0 (Plant Extracts)
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Entry Date(s):
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Date Created: 20200904 Date Completed: 20200918 Latest Revision: 20200918
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Update Code:
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20240104
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DOI:
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10.21873/anticanres.14504
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PMID:
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32878789
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Background/aim: Despite being a rare disease, melanoma is considered the most dangerous skin cancer due to its highly invasive and aggressive nature, and still requires for more effective treatments. The aim of this study was to evaluate the in vitro anti-melanoma potential of Ephedranthus pisocarpus R.E.Fr. (Annonaceae), a popular Brazilian plant with medicinal properties.
Materials and Methods: Initially, the ethanolic extract (EtOH) was obtained from E. pisocarpus leaves and later partitioned using increasing polarity solvents. The anti-melanoma potential of E. pisocarpus was assessed by spectrophotometry and its cytotoxicity determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and confocal microscopy.
Results: We demonstrated that the EtOH extract and fractions from E. pisocarpus had a moderate photoprotective action (FPS 3.0-5.0) against UVA radiation. Interestingly, the dichloromethane fraction presented higher anti-melanoma activity against B16-F10 (IC 50 =46.8 μg/ml) and SK-MEL-28 cells (IC 50 =40.1 μg/ml) and lesser toxicity on normal cells. Additionally, our study reported that spathulenol, one of the major constituents from E. pisocarpus, acts through an apoptosis-dependent mechanism in SK-MEL-28 cells.
Conclusion: The present study demonstrated, for the first time, the in vitro anti-melanoma potential of E. pisocarpus against melanoma cells.
(Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
