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Tytuł:
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Yarrowia lipolytica as a Metabolic Engineering Platform for the Production of Very-Long-Chain Wax Esters.
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Autorzy:
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Gao Q; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.
Yang JL; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.
Zhao XR; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.
Liu SC; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.
Liu ZJ; Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Key Laboratory of Industrial Microbiology, Hubei Collaborative Innovation Center of Industrial Fermentation, Hubei University of Technology, Wuhan 430068, P. R. China.
Wei LJ; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.
Hua Q; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.; Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai 200237, P. R. China.
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Źródło:
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Journal of agricultural and food chemistry [J Agric Food Chem] 2020 Sep 30; Vol. 68 (39), pp. 10730-10740. Date of Electronic Publication: 2020 Sep 19.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Washington, American Chemical Society.
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MeSH Terms:
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Esters/*metabolism
Fatty Acids/*biosynthesis
Waxes/*metabolism
Yarrowia/*genetics
Yarrowia/*metabolism
Esters/chemistry ; Fatty Acids/chemistry ; Metabolic Engineering ; Waxes/chemistry
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Contributed Indexing:
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Keywords: Yarrowia lipolytica; fatty alcohols; very-long-chain fatty acids; wax esters
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Substance Nomenclature:
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0 (Esters)
0 (Fatty Acids)
0 (Waxes)
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Entry Date(s):
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Date Created: 20200908 Date Completed: 20210204 Latest Revision: 20210204
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Update Code:
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20240105
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DOI:
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10.1021/acs.jafc.0c04393
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PMID:
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32896122
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The oleaginous yeast Yarrowia lipolytica is an attractive cell factory platform strain and can be used for sustainable production of high-value oleochemical products. Wax esters (WEs) have a good lubricating property and are usually used as a base for the production of advanced lubricants and emollient oils. In this study, we reported the metabolic engineering of Y. lipolytica to heterologously biosynthesize high-content very-long-chain fatty acids (VLCFAs) and fatty alcohols and efficiently esterify them to obtain very-long-chain WEs. Co-expression of fatty acid elongases from different sources in Y. lipolytica could yield VLCFAs with carbon chain lengths up to 24. Combining with optimization of the central metabolic modules could further enhance the biosynthesis of VLCFAs. Furthermore, through the screening of heterologous fatty acyl reductases (FARs), we enabled high-level production of fatty alcohols. Genomic integration and heterologous expression of wax synthase (WS) and FAR in a VLCFA-producing Y. lipolytica strain yielded 95-650 mg/L WEs with carbon chain lengths from 32 to 44. Scaled-up fermentation in 5 L laboratory bioreactors significantly increased the production of WEs to 2.0 g/L, the highest content so far in yeasts. This study contributes to the further efficient biosynthesis of VLCFAs and their derivatives.
