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Tytuł:
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Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Accurate Confirmatory Diagnosis of HTLV-1/2 Infection.
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Autorzy:
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Gomes Y; Laboratory for Clinical Research in Neuroinfections, Evandro Chagas National Institute of Infectious Diseases (INI), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil.
Caterino-de-Araujo A; Laboratory of HTLV Research, Immunology Center, Adolfo Lutz Institute, São Paulo 01246-000, Brazil.
Campos K; Laboratory of HTLV Research, Immunology Center, Adolfo Lutz Institute, São Paulo 01246-000, Brazil.
Gonçalves MG; Laboratory of HTLV Research, Immunology Center, Adolfo Lutz Institute, São Paulo 01246-000, Brazil.
Leite AC; Laboratory for Clinical Research in Neuroinfections, Evandro Chagas National Institute of Infectious Diseases (INI), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil.
Lima MA; Laboratory for Clinical Research in Neuroinfections, Evandro Chagas National Institute of Infectious Diseases (INI), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil.
Araújo A; Laboratory for Clinical Research in Neuroinfections, Evandro Chagas National Institute of Infectious Diseases (INI), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil.
Silva MT; Laboratory for Clinical Research in Neuroinfections, Evandro Chagas National Institute of Infectious Diseases (INI), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil.
Espíndola O; Laboratory for Clinical Research in Neuroinfections, Evandro Chagas National Institute of Infectious Diseases (INI), Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro 21040-900, Brazil.
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Źródło:
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Viruses [Viruses] 2020 Sep 04; Vol. 12 (9). Date of Electronic Publication: 2020 Sep 04.
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Typ publikacji:
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Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Original Publication: Basel, Switzerland : MDPI
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MeSH Terms:
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HTLV-I Infections/*virology
HTLV-II Infections/*virology
Human T-lymphotropic virus 1/*genetics
Human T-lymphotropic virus 2/*genetics
Molecular Diagnostic Techniques/*methods
Nucleic Acid Amplification Techniques/*methods
Blood/virology ; Clinical Laboratory Techniques ; HTLV-I Infections/blood ; HTLV-I Infections/diagnosis ; HTLV-II Infections/blood ; HTLV-II Infections/diagnosis ; Human T-lymphotropic virus 1/classification ; Human T-lymphotropic virus 1/isolation & purification ; Human T-lymphotropic virus 2/classification ; Human T-lymphotropic virus 2/isolation & purification ; Humans ; Sensitivity and Specificity
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References:
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Contributed Indexing:
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Keywords: HTLV-1; HTLV-2; LAMP; confirmatory diagnosis
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SCR Protocol:
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LAMP assay
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Entry Date(s):
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Date Created: 20200909 Date Completed: 20210309 Latest Revision: 20210309
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Update Code:
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20240105
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PubMed Central ID:
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PMC7552020
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DOI:
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10.3390/v12090981
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PMID:
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32899621
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Laboratory diagnosis of human T-lymphotropic viruses (HTLV) 1 and 2 infection is performed by serological screening and further confirmation with serological or molecular assays. Thus, we developed a loop-mediated isothermal nucleic acid amplification (LAMP) assay for the detection of HTLV-1/2 in blood samples. The sensitivity and accuracy of HTLV-1/2 LAMP were defined with DNA samples from individuals infected with HTLV-1 ( n = 125), HTLV-2 ( n = 19), and coinfected with HIV ( n = 82), and compared with real-time polymerase chain reaction (qPCR) and PCR-restriction fragment length polymorphism (RFLP). The overall accuracy of HTLV-1/2 LAMP (95% CI 74.8-85.5%) was slightly superior to qPCR (95% CI 69.5-81.1%) and similar to PCR-RFLP (95% CI 79.5-89.3%). The sensitivity of LAMP was greater for HTLV-1 (95% CI 83.2-93.4%) than for HTLV-2 (95% CI 43.2-70.8%). This was also observed in qPCR and PCR-RFLP, which was associated with the commonly lower HTLV-2 proviral load. All molecular assays tested showed better results with samples from HTLV-1/2 mono-infected individuals compared with HIV-coinfected patients, who present lower CD4 T-cell counts. In conclusion, HTLV-1/2 LAMP had similar to superior performance than PCR-based assays, and therefore may represent an attractive alternative for HTLV-1/2 diagnosis due to reduced working time and costs, and the simple infrastructure needed.
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