Small Produce Farm Environments Can Harbor Diverse Listeria monocytogenes and Listeria spp. Populations.

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Abstrakt

Tytuł:: Small Produce Farm Environments Can Harbor Diverse Listeria monocytogenes and Listeria spp. Populations.
Autorzy:: Belias A; Department of Food Science, Cornell University, 354 Stocking Hall, Ithaca, New York 14853.
Strawn LK; Department of Food Science and Technology, Eastern Shore Agricultural Research and Extension Center, Virginia Tech, 33446 Research Drive, Painter, Virginia 23420.
Wiedmann M; Department of Food Science, Cornell University, 354 Stocking Hall, Ithaca, New York 14853.
Weller D; Department of Food Science, Cornell University, 354 Stocking Hall, Ithaca, New York 14853.; (ORCID: https://orcid.org/0000-0002-6308-8025 [D.W.]).; Department of Statistics and Computational Biology, University of Rochester, Rochester, New York 14627.; Department of Environmental and Forestry Biology, SUNY College of Environmental Science and Forestry, Syracuse, New York, 13210, USA.
Źródło:: Journal of food protection [J Food Prot] 2021 Jan 01; Vol. 84 (1), pp. 113-121.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: 2023- : [New York, NY] : Elsevier
Original Publication: Ames, Iowa, International Association of Milk, Food, and Environmental Sanitarians.
MeSH Terms:: Listeria*
Listeria monocytogenes*/genetics
Electrophoresis, Gel, Pulsed-Field ; Farms ; Food Microbiology ; New York
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Grant Information:: T32 ES007271 United States ES NIEHS NIH HHS
Contributed Indexing:: Keywords: Listeria; sigB; Agricultural water; Feces; Pulsed-field gel electrophoresis
Entry Date(s):: Date Created: 20200911 Date Completed: 20210416 Latest Revision: 20230227
Update Code:: 20240105
PubMed Central ID:: PMC8000000
DOI:: 10.4315/JFP-20-179
PMID:: 32916716
: Czasopismo naukowe

Abstract: A comprehensive understanding of foodborne pathogen diversity in preharvest environments is necessary to effectively track pathogens on farms and identify sources of produce contamination. As such, this study aimed to characterize Listeria diversity in wildlife feces and agricultural water collected from a New York state produce farm over a growing season. Water samples were collected from a pond (n = 80) and a stream (n = 52). Fecal samples (n = 77) were opportunistically collected from areas <5 m from the water sources; all samples were collected from a <0.5-km2 area. Overall, 86 (41%) and 50 (24%) of 209 samples were positive for Listeria monocytogenes and Listeria spp. (excluding L. monocytogenes), respectively. For each positive sample, one L. monocytogenes or Listeria spp. isolate was speciated by sequencing the sigB gene, thereby allowing for additional characterization based on the sigB allelic type. The 86 L. monocytogenes and 50 Listeria spp. isolates represented 8 and 23 different allelic types, respectively. A subset of L. monocytogenes isolates (n = 44) from pond water and pond-adjacent feces (representing an ∼5,000-m2 area) were further characterized by pulsed-field gel electrophoresis (PFGE); these 44 isolates represented 22 PFGE types, which is indicative of considerable diversity at a small spatial scale. Ten PFGE types were isolated more than once, suggesting persistence or reintroduction of PFGE types in this area. Given the small spatial scale, the prevalence of L. monocytogenes and Listeria spp., as well as the considerable diversity among isolates, suggests traceback investigations may be challenging. For example, traceback of finished product or processing facility contamination with specific subtypes to preharvest sources may require collection of large sample sets and characterization of a considerable number of isolates. Our data also support the adage "absence of evidence does not equal evidence of absence" as applies to L. monocytogenes traceback efforts at the preharvest level.
(Published 2021 by the International Association for Food Protection.)

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