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Tytuł:
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Toxicokinetics of diisobutyl phthalate and its major metabolite, monoisobutyl phthalate, in rats: UPLC-ESI-MS/MS method development for the simultaneous determination of diisobutyl phthalate and its major metabolite, monoisobutyl phthalate, in rat plasma, urine, feces, and 11 various tissues collected from a toxicokinetic study.
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Autorzy:
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Jeong SH; College of Pharmacy, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, 61186, Republic of Korea.
Jang JH; College of Pharmacy, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, 61186, Republic of Korea.
Cho HY; College of Pharmacy, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-Do, 13488, Republic of Korea.
Lee YB; College of Pharmacy, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju, 61186, Republic of Korea. Electronic address: .
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Źródło:
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Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2020 Nov; Vol. 145, pp. 111747. Date of Electronic Publication: 2020 Sep 11.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Exeter : Elsevier Science Ltd
Original Publication: Oxford ; New York : Pergamon Press, c1982-
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MeSH Terms:
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Animal Structures/*chemistry
Dibutyl Phthalate/*analogs & derivatives
Feces/*chemistry
Phthalic Acids/*chemistry
Plasma/*chemistry
Urine/*chemistry
Animals ; Chromatography, High Pressure Liquid ; Dibutyl Phthalate/chemistry ; Dibutyl Phthalate/metabolism ; Half-Life ; Male ; Phthalic Acids/metabolism ; Rats ; Rats, Sprague-Dawley ; Risk Assessment ; Tandem Mass Spectrometry ; Toxicokinetics
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Contributed Indexing:
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Keywords: Diisobutyl phthalate; Monoisobutyl phthalate; Tissue distribution; Toxicokinetics; UPLC-ESI-MS/MS
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Substance Nomenclature:
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0 (Phthalic Acids)
0 (monoisononylphthalate)
2286E5R2KE (Dibutyl Phthalate)
IZ67FTN290 (diisobutyl phthalate)
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Entry Date(s):
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Date Created: 20200914 Date Completed: 20210514 Latest Revision: 20210514
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Update Code:
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20240105
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DOI:
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10.1016/j.fct.2020.111747
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PMID:
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32926938
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The aim of this study was to explore the toxicokinetics of diisobutyl-phthalate (DiBP) and its major metabolite, monoisobutyl-phthalate (MiBP), by developing a UPLC-ESI-MS/MS method for simultaneously measuring DiBP and MiBP in rat plasma, urine, feces, and 11 different tissues. For the experiment, 0.1% (v/v) aqueous formic acid and acetonitrile mobile phase by gradient elution at a flow rate of 0.3 mL/min, equipped with a KINETEX core-shell C 18 -column (50 × 2.1 mm, 1.7 μm), was used to completely separate analytes. The mass transitions were m/z 279.1 → 149.0 for DiBP, 221.0 → 77.0 for MiBP, and 283.2 → 153.0 for DiBP-d 4 as an internal standard. The developed assay had lower limits of quantification of 0.01 ng/mL for DiBP and 0.1 ng/mL for MiBP at all biological matrices. Toxicokinetics of DiBP were characterized by extensive distribution, short half-life, and high clearance. DiBP was rapidly metabolized to MiBP, with MiBP levels consistently exceeding the DiBP levels. Distribution of MiBP to tissues was considerable. The developed analytical method satisfied international criteria and was successfully applied to toxicokinetic studies after oral and intravenous administration of DiBP to rats. Findings of this study may be useful for evaluating the external exposure and toxic potential of DiBP and its metabolite in risk assessment.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)