A Single-Cell RNA Expression Map of Human Coronavirus Entry Factors.

Tytuł:: A Single-Cell RNA Expression Map of Human Coronavirus Entry Factors.
Autorzy:: Singh M; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA. Electronic address: .
Bansal V; Biomedical Data Science and Machine Learning Group, German Center for Neurodegenerative Diseases, Tübingen 72076, Germany; Clinical Neuroscience, Max Planck Institute of Experimental Medicine, Göttingen 37075, Germany. Electronic address: .
Feschotte C; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA. Electronic address: .
Źródło:: Cell reports [Cell Rep] 2020 Sep 22; Vol. 32 (12), pp. 108175. Date of Electronic Publication: 2020 Sep 03.
Typ publikacji:: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: [Cambridge, MA] : Cell Press, c 2012-
MeSH Terms:: Virus Internalization*
Coronavirus Infections/*pathology
Nasal Mucosa/*metabolism
Pneumonia, Viral/*pathology
Receptors, Virus/*genetics
Viral Tropism/*genetics
A549 Cells ; Angiotensin-Converting Enzyme 2 ; Animals ; Betacoronavirus/growth & development ; COVID-19 ; Cell Line ; Chlorocebus aethiops ; Enterocytes/metabolism ; Gene Expression Profiling ; Goblet Cells/metabolism ; HEK293 Cells ; Humans ; Kidney Tubules, Proximal/cytology ; Kidney Tubules, Proximal/metabolism ; Nasal Mucosa/virology ; Pandemics ; Peptidyl-Dipeptidase A/genetics ; Peptidyl-Dipeptidase A/metabolism ; SARS-CoV-2 ; Serine Endopeptidases/genetics ; Serine Endopeptidases/metabolism ; Single-Cell Analysis ; Vero Cells
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Grant Information:: R35 GM122550 United States GM NIGMS NIH HHS
Contributed Indexing:: Keywords: COVID-19; SARS-CoV-2; coronaviruses; restriction factors; scRNA-seq; viral receptors
Substance Nomenclature:: 0 (Receptors, Virus)
EC 3.4.15.1 (Peptidyl-Dipeptidase A)
EC 3.4.17.23 (ACE2 protein, human)
EC 3.4.17.23 (Angiotensin-Converting Enzyme 2)
EC 3.4.21.- (Serine Endopeptidases)
EC 3.4.21.- (TMPRSS2 protein, human)
Entry Date(s):: Date Created: 20200918 Date Completed: 20201006 Latest Revision: 20240330
Update Code:: 20240330
PubMed Central ID:: PMC7470764
DOI:: 10.1016/j.celrep.2020.108175
PMID:: 32946807
: Czasopismo naukowe

Full Text Finder

To predict the tropism of human coronaviruses, we profile 28 SARS-CoV-2 and coronavirus-associated receptors and factors (SCARFs) using single-cell transcriptomics across various healthy human tissues. SCARFs include cellular factors both facilitating and restricting viral entry. Intestinal goblet cells, enterocytes, and kidney proximal tubule cells appear highly permissive to SARS-CoV-2, consistent with clinical data. Our analysis also predicts non-canonical entry paths for lung and brain infections. Spermatogonial cells and prostate endocrine cells also appear to be permissive to SARS-CoV-2 infection, suggesting male-specific vulnerabilities. Both pro- and anti-viral factors are highly expressed within the nasal epithelium, with potential age-dependent variation, predicting an important battleground for coronavirus infection. Our analysis also suggests that early embryonic and placental development are at moderate risk of infection. Lastly, SCARF expression appears broadly conserved across a subset of primate organs examined. Our study establishes a resource for investigations of coronavirus biology and pathology.
Competing Interests: Declaration of Interests The authors declare no competing interests.
(Copyright © 2020. Published by Elsevier Inc.)

Update of: bioRxiv. 2020 May 17;:. (PMID: 32511375)
Update of: SSRN. 2020 May 27;:3611279. (PMID: 32714119)

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