-
Tytuł:
-
Bioreactor-Based Antigen Production Process Using the Baculovirus Expression Vector System.
-
Autorzy:
-
Harnischfeger J; Institute of Bioprocess Engineering and Pharmaceutical Technology (IBPT), Technische Hochschule Mittelhessen (THM) - University of Applied Sciences, Giessen, Germany.
Käßer L; Institute of Bioprocess Engineering and Pharmaceutical Technology (IBPT), Technische Hochschule Mittelhessen (THM) - University of Applied Sciences, Giessen, Germany.
Zitzmann J; Institute of Bioprocess Engineering and Pharmaceutical Technology (IBPT), Technische Hochschule Mittelhessen (THM) - University of Applied Sciences, Giessen, Germany.
Salzig D; Institute of Bioprocess Engineering and Pharmaceutical Technology (IBPT), Technische Hochschule Mittelhessen (THM) - University of Applied Sciences, Giessen, Germany.
Czermak P; Institute of Bioprocess Engineering and Pharmaceutical Technology (IBPT), Technische Hochschule Mittelhessen (THM) - University of Applied Sciences, Giessen, Germany. .; Faculty of Biology and Chemistry, Justus-Liebig-University Giessen, Giessen, Germany. .; Division Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Giessen, Germany. .
-
Źródło:
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2183, pp. 95-118.
-
Typ publikacji:
-
Journal Article; Research Support, Non-U.S. Gov't
-
Język:
-
English
-
Imprint Name(s):
-
Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,
-
MeSH Terms:
-
Batch Cell Culture Techniques*
Bioreactors*
Recombinant Proteins*
Antigens/*biosynthesis
Antigens/*genetics
Baculoviridae/*genetics
Genetic Vectors/*genetics
Animals ; Antigens/isolation & purification ; Cell Culture Techniques ; Cloning, Molecular ; Gene Expression ; Genetic Engineering ; Sf9 Cells ; Transfection ; Workflow
-
Contributed Indexing:
-
Keywords: Antigen production; Cell cultivation; Dielectric spectroscopy; Online process monitoring; Process analytical technology; Sf-9 cells; Spodoptera frugiperda
-
Substance Nomenclature:
-
0 (Antigens)
0 (Recombinant Proteins)
-
Entry Date(s):
-
Date Created: 20200922 Date Completed: 20210325 Latest Revision: 20210325
-
Update Code:
-
20240105
-
DOI:
-
10.1007/978-1-0716-0795-4_8
-
PMID:
-
32959243
-
Several vaccines are already produced using the baculovirus expression vector system (BEVS). This chapter describes methods for generating recombinant baculoviral DNA (also called bacmid) for cultivating Spodoptera frugiperda Sf-9 cells and producing a baculovirus stock from the recombinant bacmid and for producing a protein-based vaccine with the BEVS in a stirred tank reactor.
