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Tytuł pozycji:

Identification and validation of early genetic biomarkers for apple replant disease.

Tytuł:
Identification and validation of early genetic biomarkers for apple replant disease.
Autorzy:
Rohr AD; Institute of Horticultural Production Systems, Woody Plant and Propagation Physiology Section, Gottfried Wilhelm Leibniz University Hannover, Hanover, Germany.
Schimmel J; Institute of Soil Science, Gottfried Wilhelm Leibniz University Hannover, Hanover, Germany.
Liu B; Institute of Pharmaceutical Biology, Technische Universität Braunschweig, Braunschweig, Germany.
Beerhues L; Institute of Pharmaceutical Biology, Technische Universität Braunschweig, Braunschweig, Germany.
Guggenberger G; Institute of Soil Science, Gottfried Wilhelm Leibniz University Hannover, Hanover, Germany.
Winkelmann T; Institute of Horticultural Production Systems, Woody Plant and Propagation Physiology Section, Gottfried Wilhelm Leibniz University Hannover, Hanover, Germany.
Źródło:
PloS one [PLoS One] 2020 Sep 24; Vol. 15 (9), pp. e0238876. Date of Electronic Publication: 2020 Sep 24 (Print Publication: 2020).
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Original Publication: San Francisco, CA : Public Library of Science
MeSH Terms:
Genetic Markers*
Malus/*growth & development
Plant Diseases/*genetics
Gene Expression Profiling ; Gene Expression Regulation, Plant ; Malus/genetics ; Malus/metabolism ; Plant Proteins/genetics ; Plant Roots/chemistry ; Real-Time Polymerase Chain Reaction ; Sesquiterpenes/analysis ; Soil Microbiology ; Transcription Factors/genetics ; Phytoalexins
References:
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Substance Nomenclature:
0 (Genetic Markers)
0 (Plant Proteins)
0 (Sesquiterpenes)
0 (Transcription Factors)
0 (Phytoalexins)
Entry Date(s):
Date Created: 20200924 Date Completed: 20201029 Latest Revision: 20221207
Update Code:
20240105
PubMed Central ID:
PMC7514092
DOI:
10.1371/journal.pone.0238876
PMID:
32970702
Czasopismo naukowe
Apple replant disease (ARD) is a serious threat to producers of apple trees and fruits worldwide. The ARD etiology is not unraveled and managing options are either economically not applicable or environmentally harmful. Thus, interest is given in biomarkers that allow to indicate ARD situations at early time points in order to classify soils according to ARD severity but also to analyze the effectiveness to potential countermeasures. This study aimed at (i) identifying ARD biomarkers on the transcriptional level in root tissue by analyzing the expression of previously identified candidate genes in ARD soils of different origin and texture and (ii) testing the specificity of these marker genes to ARD. In vitro propagated M26 plantlets were submitted to a bio-test with three ARD soils, either untreated or disinfected by γ-irradiation. Expression of seven candidate genes identified in a previous transcriptomic study was investigated by RT-qPCR in a time course experiment. Already three days after planting, a prominent upregulation of the phytoalexin biosynthesis genes biphenyl synthase 3 (BIS3) and biphenyl 4-hydroxylase (B4Hb) was observed in the untreated ARD variants of all three soils. The phytoalexin composition in roots was comparable for all three soils and the total phytoalexin content correlated with the expression of BIS3 and B4Hb. The third promising candidate gene that was upregulated under ARD conditions was the ethylene-responsive transcription factor 1B-like (ERF1B). In a second experiment M26 plantlets were exposed to different abiotic stressors, namely heat, salt and nutrient starvation, and candidate gene expression was determined in the roots. The expression levels of BIS3 and B4Hb were highly and specifically upregulated in ARD soil, but not upon the abiotic stress conditions, whereas ERF1B also showed higher expression under heat stress. In conclusion, BIS3 and B4Hb are recommended as early ARD biomarkers due to their high expression levels and their high specificity.
Competing Interests: The authors have declared that no competing interests exist.
Erratum in: PLoS One. 2022 Aug 8;17(8):e0272900. (PMID: 35939496)
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