Monitoring Opioid Receptor Interaction in Living Cells by Bioluminescence Resonance Energy Transfer (BRET).

Szczegóły
Abstrakt

Tytuł:: Monitoring Opioid Receptor Interaction in Living Cells by Bioluminescence Resonance Energy Transfer (BRET).
Autorzy:: Baiula M; Department of Pharmacy and Biotechnology (FaBiT), University of Bologna, Bologna, Italy. .
Źródło:: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2201, pp. 35-43.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,
MeSH Terms:: Bioluminescence Resonance Energy Transfer Techniques/*methods
Receptors, Opioid, mu/*metabolism
Animals ; Carrier Proteins/metabolism ; Cell Line ; Cell Membrane/metabolism ; Energy Transfer ; Fluorescence ; Fluorescence Resonance Energy Transfer/methods ; Humans ; Luciferases, Renilla/metabolism ; Luminescent Measurements ; Signal Transduction/drug effects
Contributed Indexing:: Keywords: BRET; Enhanced yellow fluorescent protein; Opioid receptor; Receptor dimerization; Renilla luciferase
Substance Nomenclature:: 0 (Carrier Proteins)
0 (OPRM1 protein, human)
0 (Receptors, Opioid, mu)
EC 1.13.12.5 (Luciferases, Renilla)
Entry Date(s):: Date Created: 20200925 Date Completed: 20210322 Latest Revision: 20210322
Update Code:: 20240105
DOI:: 10.1007/978-1-0716-0884-5_4
PMID:: 32975787
: Czasopismo naukowe

Bioluminescence resonance energy transfer (BRET ) is a natural phenomenon that has been successfully applied for the study of protein-protein interactions, including opioid receptor oligomers. The discovery of opioid receptor homomers and heteromers has brought to the discovery of new functions and new way of signaling and trafficking; therefore, opioid receptor oligomers may be considered as novel drug targets. Fusing receptors of interest with Renilla luciferase and with a fluorescent protein (such as EYFP ) it is possible to study opioid receptor dimerization using BRET .

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