Bioluminescence Resonance Energy Transfer (BRET) to Detect the Interactions Between Kappa Opioid Receptor and Nonvisual Arrestins.

Szczegóły
Abstrakt

Tytuł:: Bioluminescence Resonance Energy Transfer (BRET) to Detect the Interactions Between Kappa Opioid Receptor and Nonvisual Arrestins.
Autorzy:: Bedini A; Department of Pharmacy and Biotechnology (FaBiT), University of Bologna, Bologna, Italy. .
Źródło:: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2201, pp. 45-58.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,
MeSH Terms:: Arrestins/*metabolism
Bioluminescence Resonance Energy Transfer Techniques/*methods
Receptors, Opioid, kappa/*metabolism
Animals ; Cell Line ; Cell Membrane/metabolism ; Energy Transfer ; Fluorescence ; Fluorescence Resonance Energy Transfer/methods ; HEK293 Cells ; Humans ; Ligands ; Luciferases, Renilla/metabolism ; Luminescent Measurements ; Protein Binding ; Receptors, G-Protein-Coupled/metabolism ; beta-Arrestin 1/metabolism ; beta-Arrestin 2/metabolism ; beta-Arrestins/metabolism
Contributed Indexing:: Keywords: BRET; Kappa-opioid receptor; Ligand-directed signaling; Nonvisual arrestins; Renilla luciferase
Substance Nomenclature:: 0 (Arrestins)
0 (Ligands)
0 (Receptors, G-Protein-Coupled)
0 (Receptors, Opioid, kappa)
0 (beta-Arrestin 1)
0 (beta-Arrestin 2)
0 (beta-Arrestins)
EC 1.13.12.5 (Luciferases, Renilla)
Entry Date(s):: Date Created: 20200925 Date Completed: 20210322 Latest Revision: 20210322
Update Code:: 20240105
DOI:: 10.1007/978-1-0716-0884-5_5
PMID:: 32975788
: Czasopismo naukowe

Bioluminescence resonance energy transfer (BRET ) is a very sensitive technique employed to study protein-protein interactions, including G-protein-coupled receptor (GPCR ) hetero- and homo-dimerization. Recently, BRET has also been used to investigate the interaction between GPCRs (e.g.: α 2 adrenergic receptor, muscarinic M2 receptor, dopaminergic D 2 receptor) and nonvisual arrestins. Within the last decade an increasing interest arose toward opioid agonists with limited activation of arrestin-dependent signaling pathways, as they are believed to be effective analgesics with reduced adverse effects. Here a BRET protocol is described to investigate interactions between the kappa opioid receptor (KOR ) and nonvisual arrestins (arrestin-2 and arrestin-3) in HEK-293 cells, both under basal conditions and after exposure to KOR ligands.

Informacja