Measurement of Macrophage Toll-Like Receptor 4 Expression After Morphine Treatment.

Szczegóły
Abstrakt

Tytuł:: Measurement of Macrophage Toll-Like Receptor 4 Expression After Morphine Treatment.
Autorzy:: Castelli M; Department of Pharmacological and Biomolecular Science, University of Milano, Milan, Italy.
Panerai A; Department of Pharmacological and Biomolecular Science, University of Milano, Milan, Italy.
Sacerdote P; Department of Pharmacological and Biomolecular Science, University of Milano, Milan, Italy. .
Franchi S; Department of Pharmacological and Biomolecular Science, University of Milano, Milan, Italy.
Źródło:: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2201, pp. 209-217.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,
MeSH Terms:: Macrophages/*drug effects
Real-Time Polymerase Chain Reaction/*methods
Toll-Like Receptor 4/*analysis
Analgesics, Opioid/immunology ; Analgesics, Opioid/pharmacology ; Animals ; Cytokines/biosynthesis ; Immune System Phenomena ; Immunity, Innate/drug effects ; Macrophage Activation/drug effects ; Macrophages/metabolism ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Morphine/adverse effects ; Morphine/metabolism ; Morphine/pharmacology ; Receptors, Opioid/immunology ; Toll-Like Receptor 4/drug effects ; Toll-Like Receptor 4/metabolism
Contributed Indexing:: Keywords: Macrophage; Morphine; Opioid receptor; RT-real-time PCR; TLR4
Substance Nomenclature:: 0 (Analgesics, Opioid)
0 (Cytokines)
0 (Receptors, Opioid)
0 (Tlr4 protein, mouse)
0 (Toll-Like Receptor 4)
76I7G6D29C (Morphine)
Entry Date(s):: Date Created: 20200925 Date Completed: 20210322 Latest Revision: 20210322
Update Code:: 20240105
DOI:: 10.1007/978-1-0716-0884-5_19
PMID:: 32975802
: Czasopismo naukowe

The immune system is a complex and finely orchestrated system, and many soluble molecules and receptors contribute to its regulation.Recent studies have suggested that many of the modulatory effects induced by morphine on innate immunity, and in particular the effects on macrophage activation and function, can be due to the modulation of an important macrophage surface receptor, the toll-like receptor (TLR), that is primarily involved in early regulatory steps. In this chapter we describe a RT-real-time PCR method for assessing TLR expression in macrophage after in vivo morphine treatment.

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