-
Tytuł:
-
Temperature stress response: A novel important function of Dermatophagoides farinae allergens.
-
Autorzy:
-
Niu D; Department of Pathogen Biology and Immunology, School of Basic Medical Sciences, Xi'an Jiaotong University, Xi'an, 710061, China.
Zhao Y; Department of Pathogen Biology and Immunology, School of Basic Medical Sciences, Xi'an Jiaotong University, Xi'an, 710061, China. Electronic address: .
Zhang W; Department of Pathogen Biology and Immunology, School of Basic Medical Sciences, Xi'an Jiaotong University, Xi'an, 710061, China.
-
Źródło:
-
Experimental parasitology [Exp Parasitol] 2020 Nov; Vol. 218, pp. 108003. Date of Electronic Publication: 2020 Sep 24.
-
Typ publikacji:
-
Journal Article
-
Język:
-
English
-
Imprint Name(s):
-
Publication: Orlando, FL : Academic Press
Original Publication: New York.
-
MeSH Terms:
-
Antigens, Dermatophagoides/*physiology
Cold-Shock Response/*physiology
Dermatophagoides farinae/*physiology
Heat-Shock Response/*physiology
Stress, Physiological/*physiology
Animals ; Antigens, Dermatophagoides/genetics ; Antigens, Dermatophagoides/metabolism ; Antigens, Dermatophagoides/pharmacology ; Base Sequence ; Dermatophagoides farinae/genetics ; Female ; Gene Silencing ; Molecular Sequence Annotation ; RNA/chemistry ; RNA/isolation & purification ; RNA Interference/physiology ; RNA, Double-Stranded/chemistry ; Real-Time Polymerase Chain Reaction ; Seasons ; Stress, Physiological/genetics ; Up-Regulation
-
Contributed Indexing:
-
Keywords: Allergens; Dermatophagoides farinae; RNAi; Temperature stress response; qRT-PCR
-
Substance Nomenclature:
-
0 (Antigens, Dermatophagoides)
0 (RNA, Double-Stranded)
63231-63-0 (RNA)
-
Entry Date(s):
-
Date Created: 20200927 Date Completed: 20201109 Latest Revision: 20201109
-
Update Code:
-
20240105
-
DOI:
-
10.1016/j.exppara.2020.108003
-
PMID:
-
32980317
-
Dermatophagoides farinae, an important pathogen, has multiple allergens. However, their expression under physiological conditions are not understood. Our previous RNA-seq showed that allergens of D. farinae were up-regulated under temperature stress, implying that they may be involved in stress response. Here, we performed a comprehensive study. qRT-PCR detection indicated that 26 of the 34 allergens showed differential expression. Der f1 had the most abundant basic expression quantity. Der f 28.0201 (HSP70) and Der f3 had the same regulation pattern in 9 highly expressed transcripts, which only up-regulated at 41 °C and 43 °C, but Der f 28.0201 showed stronger regulation than Der f 3 (19.88-fold vs 6.02-fold). Whereas Der f 1, 2, 7, 21, 22, 27, and 30 were up-regulated under both heat and cold stress, and Der f 27 showed the strongest regulation ability among them. Der f 27 showed more significant up-regulation than Der f 28.0201 under heat stress (23.59-fold vs 19.88-fold), and Der f27 had more obvious up-regulation under cold than heat stress (30.70-fold vs 23.59-fold). The expression of Der f 27, 28.0201 and 1, and D. farinae survival rates significantly decreased following RNAi, indicating the upregulation of these allergens under temperature stress conferred thermo-tolerance or cold-tolerance to D. farinae. In this study, we described for the first time that these allergens have temperature-stress response functions. This new scientific discovery has important clinical value for revealing the more frequent and serious allergic diseases caused by D. farinae during the change of seasons.
(Copyright © 2020 Elsevier Inc. All rights reserved.)