[Influence of oxidative/antioxidative biomarkers and inflammatory cytokines on rats after sub-acute orally administration of titanium dioxide nanoparticles].

Szczegóły
Abstrakt

Tytuł:: [Influence of oxidative/antioxidative biomarkers and inflammatory cytokines on rats after sub-acute orally administration of titanium dioxide nanoparticles].
Autorzy:: Zhou D
Chen ZJ
Hu GP
Yan TL
Long CM
Feng HM
Jia G
Źródło:: Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences [Beijing Da Xue Xue Bao Yi Xue Ban] 2020 Oct 18; Vol. 52 (5), pp. 821-827.
Typ publikacji:: Journal Article
Język:: Chinese
Imprint Name(s):: Original Publication: Beijing : Beijing da xue, 2001-
MeSH Terms:: Antioxidants*
Nanoparticles*
Animals ; Biomarkers ; Cytokines ; Oxidative Stress ; Rats ; Rats, Sprague-Dawley ; Titanium
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Contributed Indexing:: Keywords: Antioxidants; Nanoparticles; Rats, Sprague-Dawley; Titanium dioxide
Substance Nomenclature:: 0 (Antioxidants)
0 (Biomarkers)
0 (Cytokines)
15FIX9V2JP (titanium dioxide)
D1JT611TNE (Titanium)
Entry Date(s):: Date Created: 20201013 Date Completed: 20201014 Latest Revision: 20201113
Update Code:: 20240105
PubMed Central ID:: PMC7653415
PMID:: 33047714
: Czasopismo naukowe

Objective: To evaluate the sub-acute oral effect of titanium dioxide (TiO 2 ) nanoparticles on the oxidation/antioxidation biomarkers and inflammatory cytokines in blood, liver, intestine, and colon in rats.
Methods: Twenty four 4-week-old clean-grade Sprague Dawley (SD) rats were randomly devided into 4 groups by body weight ( n =6, control, low, middle, and high), in which the rats were orally exposed to TiO 2 nanoparticles at doses of 0, 2, 10 and 50 mg/kg body weight/day for 28 consecutive days separately. Food intake, body weight and abnormal behaviors during the experiment were recorded. The rats were euthanized on the 29th day. The blood was collected via abdominal aortic method and centrifuged to collect the serum. Tissues from liver, intestine and colon were collected and homogenated. Then enzyme-linked immunosorbent assay (ELISA) and microwell plate methods were used to detect oxidation/antioxidation biomarkers including superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GSH-Px), total mercapto (T-SH), glutathione disulfide (GSSG), malomdialdehvde (MDA) and inflammatory cytokines including interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) in the serum, liver, intestine and colon in the rats.
Results: Compared with the control group, no significant differences in body weight, food intake and organ coefficients were observed in all the three groups after TiO 2 gavage. No significant changes in GSH, GSH-Px, T-SH, and IL-6 were observed. Compared with the control group, significant increase of SOD activity in serum in high dose group, signi-ficant increase of GSSG concentration in intestine in middle and high dose group and significant increase of MDA concentration in liver in low and high dose group were observed. Compared with the control group, a significant increase of TNF-α in liver in middle and high dose group was observed.
Conclusion: TiO 2 nanoparticle can increase antioxidant enzymes activities in blood, increase oxidative biomarkers in liver and intestine, increase inflammatory cytokines in liver in rats after a 28-day sub-acute orally administration. Among blood, liver, intestine, and colon, liver is most sensitive to the toxicity induced by TiO 2 nanoparticles, followed by intestine, blood, and colon in sequence.

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