-
Tytuł:
-
Interplay of MPP5a with Rab11 synergistically builds epithelial apical polarity and zonula adherens.
-
Autorzy:
-
Hao Y; Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China.; The Institute of Translational Medicine, Zhejiang University, Hangzhou 310058, China.
Zhou Y; Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China.; The Institute of Translational Medicine, Zhejiang University, Hangzhou 310058, China.
Yu Y; Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China.
Zheng M; Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China.; The Institute of Translational Medicine, Zhejiang University, Hangzhou 310058, China.
Weng K; Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China.; The Institute of Translational Medicine, Zhejiang University, Hangzhou 310058, China.
Kou Z; Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China.; The Institute of Translational Medicine, Zhejiang University, Hangzhou 310058, China.
Liang J; Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China.; The Institute of Translational Medicine, Zhejiang University, Hangzhou 310058, China.
Zhang Q; Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou 310058, China.
Tang X; Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China.
Xu P; Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou 310058, China.
Link BA; Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, WI 53226, USA.
Yao K; Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China .; Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou 310058, China.
Zou J; Eye Center of the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China .; The Institute of Translational Medicine, Zhejiang University, Hangzhou 310058, China.; Zhejiang Provincial Key Lab of Ophthalmology, Hangzhou 310058, China.
-
Źródło:
-
Development (Cambridge, England) [Development] 2020 Nov 19; Vol. 147 (22). Date of Electronic Publication: 2020 Nov 19.
-
Typ publikacji:
-
Journal Article; Research Support, Non-U.S. Gov't
-
Język:
-
English
-
Imprint Name(s):
-
Publication: Cambridge Eng : Company Of Biologists Limited
Original Publication: [Cambridge] : Company of Biologists, [c1987-
-
MeSH Terms:
-
Adherens Junctions/*metabolism
Cell Movement/*physiology
Cell Polarity/*physiology
Guanylate Cyclase/*metabolism
Zebrafish/*embryology
Zebrafish Proteins/*metabolism
rab GTP-Binding Proteins/*metabolism
Adherens Junctions/genetics ; Animals ; Cadherins/genetics ; Cadherins/metabolism ; Epithelial Cells ; Golgi Apparatus/genetics ; Golgi Apparatus/metabolism ; Guanylate Cyclase/genetics ; Protein Transport/physiology ; Zebrafish/genetics ; Zebrafish Proteins/genetics ; rab GTP-Binding Proteins/genetics
-
Contributed Indexing:
-
Keywords: Adherens junctions remodeling; Apical polarity; MPP5a; Rab11; Zonula adherens
-
Substance Nomenclature:
-
0 (Cadherins)
0 (Zebrafish Proteins)
EC 2.7.4.8 (mpp5a protein, zebrafish)
EC 3.6.1.- (rab11 protein)
EC 3.6.5.2 (rab GTP-Binding Proteins)
EC 4.6.1.2 (Guanylate Cyclase)
-
Entry Date(s):
-
Date Created: 20201016 Date Completed: 20210119 Latest Revision: 20240229
-
Update Code:
-
20240229
-
DOI:
-
10.1242/dev.184457
-
PMID:
-
33060129
-
Adherens junction remodeling regulated by apical polarity proteins constitutes a major driving force for tissue morphogenesis, although the precise mechanism remains inconclusive. Here, we report that, in zebrafish, the Crumbs complex component MPP5a interacts with small GTPase Rab11 in Golgi to transport cadherin and Crumbs components synergistically to the apical domain, thus establishing apical epithelial polarity and adherens junctions. In contrast, Par complex recruited by MPP5a is incapable of interacting with Rab11 but might assemble cytoskeleton to facilitate cadherin exocytosis. In accordance, dysfunction of MPP5a induces an invasive migration of epithelial cells. This adherens junction remodeling pattern is frequently observed in zebrafish lens epithelial cells and neuroepithelial cells. The data identify an unrecognized MPP5a-Rab11 complex and describe its essential role in guiding apical polarization and zonula adherens formation in epithelial cells.
Competing Interests: Competing interestsThe authors declare no competing or financial interests.
(© 2020. Published by The Company of Biologists Ltd.)
