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Tytuł:
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HPLC-UV-Based Simultaneous Determination of Canthin-6-One Alkaloids, Quassinoids, and Scopoletin: The Active Ingredients in Eurycoma Longifolia Jack and Eurycoma Harmandiana Pierre, and Their Anti-Inflammatory Activities.
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Autorzy:
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Chaingam J; Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.; Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen 40002, Thailand.
Juengwatanatrakul T; Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Ubon Ratchathani 34190, Thailand.
Yusakul G; School of Pharmacy, Walailak University, 222 Thaiburi, Thasala, Nakhon Si Thammarat 80160, Thailand.
Kanchanapoom T; Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
Putalun W; Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.; Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen 40002, Thailand.
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Źródło:
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Journal of AOAC International [J AOAC Int] 2021 Jun 12; Vol. 104 (3), pp. 802-810.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: 2020- : Oxford : Oxford University Press
Original Publication: Arlington, VA : AOAC International, c1992-
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MeSH Terms:
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Alkaloids*/pharmacology
Eurycoma*
Quassins*/pharmacology
Anti-Inflammatory Agents/pharmacology ; Carbolines ; Chromatography, High Pressure Liquid ; Humans ; Indole Alkaloids ; Male ; Plant Extracts/pharmacology ; Plant Roots ; Scopoletin
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Substance Nomenclature:
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0 (Alkaloids)
0 (Anti-Inflammatory Agents)
0 (Carbolines)
0 (Indole Alkaloids)
0 (Plant Extracts)
0 (Quassins)
3FK17S759N (canthin-6-one)
KLF1HS0SXJ (Scopoletin)
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Entry Date(s):
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Date Created: 20201016 Date Completed: 20210625 Latest Revision: 20220531
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Update Code:
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20240105
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DOI:
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10.1093/jaoacint/qsaa141
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PMID:
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33064798
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Background: Quassinoids and canthin-6-one alkaloids are bioactive markers of Eurycoma longifolia (EL) and E. harmandiana (EH) and have been commercially utilized to treat inflammation and male infertility.
Objectives: This study aims to reveal the contents of bioactive compounds and compare anti-inflammatory activities of these two species.
Methods: HPLC methods coupled with UV-Vis detection were developed and validated for the simultaneous analysis of the chemical profiles and their contents in EL and EH. The anti-inflammatory activities of both species were investigated using RAW 264.7 cell line.
Results: The HPLC methods provided a sensitivity (LOD) of 0.02-0.05 µg/mL for the eight bioactive compounds (canthin-6-one alkaloids, quassinoids, and scopoletin) with high precision (% relative standard deviation (RSD) ≤6.48) and recoveries between 80.0 and 120%. The chaparrinone: eurycomanone ratio was high in EH, whereas EL had a higher ratio of eurycomanone: chaparrinone than EH. The contents of total canthin-6-one alkaloids, quassinoids, and scopoletin were 0.01-0.75, 0.19-1.54, and 0.01-0.28 mg/g, respectively, in EL roots and 0.12-1.80, 7.05-9.26, and 0.02 mg/g, respectively, in EH roots. The anti-inflammatory effects of EL and EH extracts varied among the samples due to the variation in their chemical constituents.
Conclusions: In summary, our study indicated that chaparrinone was the major compound in EH. EH exhibited anti-inflammatory activity to the same extent as EL.
Highlights: EH and EL extracts were analyzed using developed HPLC-UV methods, revealing a high concentration of chaparrinone in EH, and an anti-inflammatory assay indicated that EH had a potency comparable to that of EL.
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