N,N'-Diphenylthiourea electrochemical sensor for the detection of l-glutamate and Aspartate in beef.

Szczegóły
Abstrakt

Tytuł:: N,N'-Diphenylthiourea electrochemical sensor for the detection of l-glutamate and Aspartate in beef.
Autorzy:: Wang X; College of Food Science and Engineering, Jilin University, Changchun, P. R. China.
Hu F; College of Food Science and Engineering, Jilin University, Changchun, P. R. China.
Zhu L; College of Food Science and Engineering, Jilin University, Changchun, P. R. China.
Liu D; College of Food Science and Technology, Bohai University, Jinzhou, P. R. China.
Dong Y; College of Food Science and Engineering, Jilin University, Changchun, P. R. China.
Wang C; College of Food Science and Engineering, Jilin University, Changchun, P. R. China.
Wu D; College of Food Science and Engineering, Jilin University, Changchun, P. R. China.
Źródło:: Journal of food science [J Food Sci] 2020 Nov; Vol. 85 (11), pp. 3852-3857. Date of Electronic Publication: 2020 Oct 16.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: Malden, Mass. : Wiley on behalf of the Institute of Food Technologists
Original Publication: Champaign, Ill. Institute of Food Technologists
MeSH Terms:: Aspartic Acid/*analysis
Electrochemical Techniques/*methods
Glutamic Acid/*analysis
Red Meat/*analysis
Thiourea/*analogs & derivatives
Animals ; Cattle ; Reproducibility of Results ; Thiourea/analysis
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Grant Information:: 2019-MS-006 Natural Science Foundation of Liaoning Province
Contributed Indexing:: Keywords: DPTU; aspartate; beef; electrochemical sensor; l-glutamate
Substance Nomenclature:: 30KYC7MIAI (Aspartic Acid)
3KX376GY7L (Glutamic Acid)
9YCB5VR86Z (diphenylthiourea)
GYV9AM2QAG (Thiourea)
Entry Date(s):: Date Created: 20201017 Date Completed: 20210108 Latest Revision: 20210108
Update Code:: 20240105
DOI:: 10.1111/1750-3841.15494
PMID:: 33067859
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This study was conducted to design an electrochemical sensor for detection of l-glutamate (L-Glu) and Aspartate (Asp), which contribute largely to the umami taste of beef. Using N, N'-diphenylthiourea (DPTU), polypyrrole (PPy), and polyvinyl chloride (PVC), a composite electrode (DPTU/PVC/PPy/Pt) was prepared for rapidly electrochemical detection of l-Glu and Asp. The surface morphology of the synthesized functionalized electrode was characterized by Field Emission Scanning Electron Microscopy (FESEM). Potentials of umami amino acids accounted for 97.8%, while six interferential amino acids existed. The linear correlation between the content of l-Glu and Asp in beef broth was studied under different treatment conditions. The sensor compared with an amino acid analyzer well detected the contents of l-Glu and Asp in beef broths, with a coefficient of 0.991 in Pearson correlation analysis and an accuracy of 88.9%. The proposed electrochemical sensor showed good concurrence with previously reported methods and was effectively employed for the quantification of l- Glu and Asp in beef. PRACTICAL APPLICATION: The sensor exhibits the good performance with high stability and high accuracy. And it is a potential sensing platform with good reproducibility, making the proposed method suitable and reliable for routine analysis of L-Glu and Asp in beef. This method was proved promising for quantitative detecting amino acids in beef.
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