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Tytuł:
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Alteration of the goat milk glycoproteins N/O-glycome at different lactation stages.
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Autorzy:
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Lu Y; The College of Life Sciences, Northwest University, Xi'an 710069, China.
Li C; Shaanxi Natural Carbohydrate Resource Engineering Research Center, College of Food Science and Technology, Northwest University, Xi'an 710069, China.
Liu J; The College of Life Sciences, Northwest University, Xi'an 710069, China.
Huang W; Shaanxi Natural Carbohydrate Resource Engineering Research Center, College of Food Science and Technology, Northwest University, Xi'an 710069, China.
Yang Y; The College of Life Sciences, Northwest University, Xi'an 710069, China.
Jia Y; The College of Life Sciences, Northwest University, Xi'an 710069, China.
Liu T; The College of Life Sciences, Northwest University, Xi'an 710069, China.
Huang L; The College of Life Sciences, Northwest University, Xi'an 710069, China; Shaanxi Natural Carbohydrate Resource Engineering Research Center, College of Food Science and Technology, Northwest University, Xi'an 710069, China. Electronic address: .
Wang Z; The College of Life Sciences, Northwest University, Xi'an 710069, China; Shaanxi Natural Carbohydrate Resource Engineering Research Center, College of Food Science and Technology, Northwest University, Xi'an 710069, China. Electronic address: .
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Źródło:
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Food chemistry [Food Chem] 2021 Apr 16; Vol. 342, pp. 128221. Date of Electronic Publication: 2020 Oct 12.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: Barking : Elsevier Applied Science Publishers
Original Publication: Barking, Eng., Applied Science Publishers.
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MeSH Terms:
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Goats*
Lactation*
Glycoproteins/*chemistry
Glycoproteins/*metabolism
Animals ; Colostrum/metabolism ; Female ; Glycosylation ; Humans ; Pregnancy
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Contributed Indexing:
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Keywords: ESI-MS; Goat milk glycoproteins; Lactation stages; N/O-glycome; Online LC-MS/MS
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Substance Nomenclature:
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0 (Glycoproteins)
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Entry Date(s):
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Date Created: 20201023 Date Completed: 20210210 Latest Revision: 20210210
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Update Code:
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20240105
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DOI:
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10.1016/j.foodchem.2020.128221
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PMID:
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33092920
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Goat milk oligosaccharides represent an unexplored multi-functional ingredient for the dairy industry. Here, we qualitatively and quantitatively compared the N/O-glycome at different lactation stages via online hydrophilic interaction chromatography-tandem mass spectrometry. Complex N-glycans and high mannose N-glycans constituted 82.1% and 17.9% of the glycan pool, respectively. N-glycans with isomers containing non-bisected antenna complex structures accounted for 30.8%. N-glycans modified with Neu5Ac, Neu5Gc and fucosylated were 3.7%, 5.3% and 35.3%. The triantennary trifucosylated complex N-glycan (H5N5F3) was reported for the first time. A comparison between colostrum and mature milk revealed a 1.20-fold decrease in total N-glycans and 1.66-fold decrease in fucosylation with ongoing lactation, echoing the trend in human milk. Similarly, Neu5Ac- and Neu5Gc-modified sialylation decreased by 1.69 and 3.62 times, respectively. In the O-glycome, 46.2% of structures were O-linked core 1, 23.1% were O-linked core 2, 7.7% were O-linked core 3 and core 4. As lactation progressed, overall O-glycans content decreased by 1.26-fold. Unlike human milk, Neu5Ac- and Neu5Gc-modified sialylation increased by 4.4 and 2 times, respectively. These findings will facilitate research on the structure-function relationship of goat milk oligosaccharides and the development of formula food targeting different age groups.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
