Exposure to small molecule cocktails allows induction of neural crest lineage cells from human adipose-derived mesenchymal stem cells.

Tytuł:: Exposure to small molecule cocktails allows induction of neural crest lineage cells from human adipose-derived mesenchymal stem cells.
Autorzy:: Takayama Y; Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
Akagi Y; Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.; Advanced Photonics and Biosensing Open Innovation Laboratory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
Shibuya Y; Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.; Department of Plastic and Reconstructive Surgery, University of Tsukuba, Tsukuba, Ibaraki, Japan.
Kida YS; Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.; Advanced Photonics and Biosensing Open Innovation Laboratory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
Źródło:: PloS one [PLoS One] 2020 Oct 26; Vol. 15 (10), pp. e0241125. Date of Electronic Publication: 2020 Oct 26 (Print Publication: 2020).
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: San Francisco, CA : Public Library of Science
MeSH Terms:: Cell Culture Techniques*
Culture Media*
Mesenchymal Stem Cells*/cytology
Mesenchymal Stem Cells*/metabolism
Neural Crest*/cytology
Neural Crest*/metabolism
Regenerative Medicine/*methods
CD57 Antigens/metabolism ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Humans ; Nerve Tissue Proteins/metabolism ; Receptors, Nerve Growth Factor/metabolism ; SOXE Transcription Factors/metabolism
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Substance Nomenclature:: 0 (CD57 Antigens)
0 (Culture Media)
0 (NGFR protein, human)
0 (Nerve Tissue Proteins)
0 (Receptors, Nerve Growth Factor)
0 (SOX10 protein, human)
0 (SOXE Transcription Factors)
Entry Date(s):: Date Created: 20201026 Date Completed: 20201217 Latest Revision: 20201217
Update Code:: 20240105
PubMed Central ID:: PMC7588063
DOI:: 10.1371/journal.pone.0241125
PMID:: 33104750
: Czasopismo naukowe

Pełny tekst

Neural crest cells (NCCs) are a promising source for cell therapy and regenerative medicine owing to their multipotency, self-renewability, and capability to secrete various trophic factors. However, isolating NCCs from adult organs is challenging, because NCCs are broadly distributed throughout the body. Hence, we attempted to directly induce NCCs from human adipose-derived mesenchymal stem cells (ADSCs), which can be isolated easily, using small molecule cocktails. We established a controlled induction protocol with two-step application of small molecule cocktails for 6 days. The induction efficiency was evaluated based on mRNA and protein expression of neural crest markers, such as nerve growth factor receptor (NGFR) and sex-determining region Y-box 10 (SOX10). We also found that various trophic factors were significantly upregulated following treatment with the small molecule cocktails. Therefore, we performed global profiling of cell surface makers and identified distinctly upregulated markers, including the neural crest-specific cell surface markers CD271 and CD57. These results indicate that our chemical treatment can direct human ADSCs to developing into the neural crest lineage. This offers a promising experimental platform to study human NCCs for applications in cell therapy and regenerative medicine.
Competing Interests: I have read the journal’s policy and the authors of this manuscript have the following competing interests: YSK was funded by BioMimetics Sympathies Inc. This does not alter the authors’ adherence to PLOS ONE policies on sharing data and materials.

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