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Tytuł:
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Proteomics Study on the Differentially Expressed Proteins in c-fos-silenced Cells Exposed to PM 2.5 .
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Autorzy:
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Cai Y; School of Public Health, University of South China, Hengyang 421001, Hunan, China;Institute of Environment and Health, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, Guangdong, China.
Zheng K; School of Public Health, University of South China, Hengyang 421001, Hunan, China;Institute of Environment and Health, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, Guangdong, China.
Li RB; School of Public Health, University of South China, Hengyang 421001, Hunan, China;Institute of Environment and Health, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, Guangdong, China.
Yu SY; Institute of Environment and Health, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, Guangdong, China.
Liu N; Institute of Environment and Health, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, Guangdong, China.
Ji JJ; Institute of Environment and Health, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, Guangdong, China.
Yang C; Institute of Environment and Health, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, Guangdong, China.
Wu S; Institute of Environment and Health, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, Guangdong, China.
Qin SJ; Institute of Environment and Health, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, Guangdong, China;Xiangya School of Public Health, Central South University, Changsha 410078, Hunan, China.
Li BR; Institute of Environment and Health, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, Guangdong, China;Xiangya School of Public Health, Central South University, Changsha 410078, Hunan, China.
Zhang ZH; School of Public Health, University of South China, Hengyang 421001, Hunan, China.
Xu XY; Institute of Environment and Health, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, Guangdong, China.
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Źródło:
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Biomedical and environmental sciences : BES [Biomed Environ Sci] 2020 Sep 20; Vol. 33 (9), pp. 680-689.
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Publication: [Beijing, China] : Chinese Center for Disease Control and Prevention
Original Publication: Duluth, MN : Academic Press, [c1988-
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MeSH Terms:
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Gene Expression Regulation*
Gene Silencing*
Air Pollutants/*toxicity
Bronchi/*drug effects
Genes, fos/*genetics
Particulate Matter/*toxicity
Respiratory Mucosa/*drug effects
Bronchi/metabolism ; Cells, Cultured ; Humans ; Proteomics ; Respiratory Mucosa/metabolism
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Contributed Indexing:
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Keywords: Bioinformatics; Gene silence; Human bronchial epithelial cells; PM2.5; Proteomics
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Substance Nomenclature:
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0 (Air Pollutants)
0 (Particulate Matter)
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Entry Date(s):
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Date Created: 20201027 Date Completed: 20201106 Latest Revision: 20220417
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Update Code:
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20240105
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DOI:
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10.3967/bes2020.089
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PMID:
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33106213
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Objective: To investigate the effect of c-fos gene silencing on differentially expressed proteins (DEPs) in human bronchial epithelial (HBE) cells after exposure to fine particulate matter (PM 2.5 ).
Methods: HBE cells and c-fos-silenced HBE cells were exposed to 50 μg/mL PM 2.5 , LC-MS/MS and tandem mass tag (TMT) labeling methods were combined with bioinformatics methods, and DEPs and interaction networks were identified.
Results: In the HBE group, 414 DEPs were screened, of which 227 were up-regulated and 187 down-regulated. In the c-fos silenced HBE group, 480 DEPs were screened, including 240 up-regulated proteins and 240 down-regulated proteins. KEGG annotations showed that DEPs in the HBE group are mainly concentrated in the glycolysis/gluconeogenesis pathway and those in the c-fos silenced group are concentrated mainly in endoplasmic reticulum and the processing of proteins. Additionally, the abnormal expression of GPRC5C, DKK4, and UBE2C was identified in top 15 DEPs. After constructing the protein interaction network, 20 Hub proteins including HNRNPA2B1, HNRNPL, RPS15A, and RPS25 were screened from the HBE group and the c-fos silenced HBE group.
Conclusion: c-fos gene affected the expression of cancer-related proteins. Our results provided a scientific basis for further study of PM 2.5 -induced carcinogenesis mechanism.
(Copyright © 2020 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.)