Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Przeglądasz jako GOŚĆ
Tytuł pozycji:

Kidney Subcapsular Allograft Transplants as a Model to Test Virus-Derived Chemokine-Modulating Proteins as Therapeutics.

Tytuł :
Kidney Subcapsular Allograft Transplants as a Model to Test Virus-Derived Chemokine-Modulating Proteins as Therapeutics.
Autorzy :
Burgin M; Centers for Personalized Diagnostics and for Immunotherapy, Vaccines and Virotherapy, Biodesign Institute, Arizona State University, Tempe, AZ, USA. .
Yaron JR; Centers for Personalized Diagnostics and for Immunotherapy, Vaccines and Virotherapy, Biodesign Institute, Arizona State University, Tempe, AZ, USA.
Zhang L; Centers for Personalized Diagnostics and for Immunotherapy, Vaccines and Virotherapy, Biodesign Institute, Arizona State University, Tempe, AZ, USA.
Guo Q; Centers for Personalized Diagnostics and for Immunotherapy, Vaccines and Virotherapy, Biodesign Institute, Arizona State University, Tempe, AZ, USA.; Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Daggett J; DACT, Biodesign Institute, Ariizona State University, Tempe, AZ, USA.
Kilbourne J; DACT, Biodesign Institute, Ariizona State University, Tempe, AZ, USA.
Lowe KM; DACT, Biodesign Institute, Ariizona State University, Tempe, AZ, USA.
Lucas AR; Centers for Personalized Diagnostics and for Immunotherapy, Vaccines and Virotherapy, Biodesign Institute, Arizona State University, Tempe, AZ, USA.; Cardiology, Saint Joseph's Hospital, Dignity Health, Phoenix, AZ, USA.
Pokaż więcej
Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2225, pp. 257-273.
Typ publikacji :
Journal Article
Język :
English
Imprint Name(s) :
Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,
MeSH Terms :
Graft Survival*
Anti-Inflammatory Agents/*pharmacology
Graft Rejection/*prevention & control
Immunologic Factors/*pharmacology
Kidney Transplantation/*methods
Myxoma virus/*chemistry
Viral Proteins/*pharmacology
Animals ; Anti-Inflammatory Agents/immunology ; Anti-Inflammatory Agents/metabolism ; Biomarkers/analysis ; Chemokines/biosynthesis ; Complement C4b/genetics ; Complement C4b/immunology ; Female ; Gene Expression ; Graft Rejection/genetics ; Graft Rejection/immunology ; Graft Rejection/pathology ; Immunologic Factors/biosynthesis ; Immunologic Factors/immunology ; Kidney/immunology ; Kidney/surgery ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Peptide Fragments/genetics ; Peptide Fragments/immunology ; Receptors, Interferon/biosynthesis ; Receptors, Interferon/immunology ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/immunology ; Recombinant Proteins/pharmacology ; Transplantation, Homologous ; Viral Proteins/biosynthesis ; Viral Proteins/immunology
Contributed Indexing :
Keywords: Immunomodulatory*; Rejection*; Renal*; Therapeutics*; Transplant*; Viral protein*
Substance Nomenclature :
0 (Anti-Inflammatory Agents)
0 (Biomarkers)
0 (Chemokines)
0 (Immunologic Factors)
0 (M-T7 protein, Myxoma virus)
0 (Peptide Fragments)
0 (Receptors, Interferon)
0 (Recombinant Proteins)
0 (Viral Proteins)
80295-50-7 (Complement C4b)
80295-52-9 (complement C4d)
Entry Date(s) :
Date Created: 20201027 Date Completed: 20210326 Latest Revision: 20210326
Update Code :
20210327
DOI :
10.1007/978-1-0716-1012-1_15
PMID :
33108668
Czasopismo naukowe
Solid tissue transplant is a growing medical need that is further complicated by a limited donor organ supply. Acute and chronic rejection occurs in nearly all transplants and reduces long-term graft survival, thus increasing the need for repeat transplantation. Viruses have evolved highly adapted responses designed to evade the host's immune defenses. Immunomodulatory proteins derived from viruses represent a novel class of potential therapeutics that are under investigation as biologics to attenuate immune-mediated rejection and damage. These immune-modulating proteins have the potential to reduce the need for traditional posttransplant immune suppressants and improve graft survival. The myxoma virus-derived protein M-T7 is a promising biologic that targets chemokine and glycosaminoglycan pathways central to kidney transplant rejection. Orthotopic transplantations in mice are prohibitively difficult and costly and require a highly trained microsurgeon to successfully perform the procedure. Here we describe a kidney-to-kidney subcapsular transplant model as a practical and simple method for studying transplant rejection, a model that requires fewer mice. One kidney can be used as a donor for transplants into six or more recipient mice. Using this model there is lower morbidity, pain, and mortality for the mice. Subcapsular kidney transplantation provides a first step approach to testing virus-derived proteins as new potential immune-modulating therapeutics to reduce transplant rejection and inflammation.

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies