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Tytuł pozycji:

Necessity for detection of SARS-CoV-2 RNA in multiple types of specimens for the discharge of the patients with COVID-19.

Tytuł:
Necessity for detection of SARS-CoV-2 RNA in multiple types of specimens for the discharge of the patients with COVID-19.
Autorzy:
Tong Y; Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Bao A; Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Chen H; Department of Pulmonary and Critical Care Medicine of Renmin Hospital, Wuhan University, Wuhan, 430060, China.
Huang J; Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Lv Z; Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Feng L; Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Cheng Y; Department of Pulmonary and Critical Care Medicine of Renmin Hospital, Wuhan University, Wuhan, 430060, China.
Wang Y; Department of Pulmonary and Critical Care Medicine of Renmin Hospital, Wuhan University, Wuhan, 430060, China.
Bai L; Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Rao W; Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Zheng H; Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Wu Z; Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Qiao B; Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Zhao Z; Clinical Laboratory Center & Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, General Hospital of Ningxia Medical University, Yinchuan, 750004, China. .
Wang H; Department of Nephrology, Renmin Hospital of Wuhan University, Wuhan, 430060, China. .
Li Y; Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, 430060, China. .
Źródło:
Journal of translational medicine [J Transl Med] 2020 Nov 02; Vol. 18 (1), pp. 411. Date of Electronic Publication: 2020 Nov 02.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
Język:
English
Imprint Name(s):
Original Publication: [London] : BioMed Central, 2003-
MeSH Terms:
Patient Discharge*
Clinical Laboratory Techniques/*methods
Coronavirus Infections/*diagnosis
Nasal Cavity/*virology
Pneumonia, Viral/*diagnosis
RNA, Viral/*blood
Betacoronavirus/isolation & purification ; Body Fluids ; COVID-19 ; COVID-19 Testing ; Hospitalization ; Humans ; Pandemics ; Real-Time Polymerase Chain Reaction ; Reproducibility of Results ; SARS-CoV-2
References:
Emerg Microbes Infect. 2020 Dec;9(1):469-473. (PMID: 32102625)
J Med Virol. 2020 Apr 24;:. (PMID: 32330305)
Emerg Microbes Infect. 2020 Dec;9(1):542-544. (PMID: 32148172)
J Med Virol. 2020 Apr 9;:. (PMID: 32270882)
Nat Microbiol. 2020 Apr;5(4):536-544. (PMID: 32123347)
J Clin Med. 2020 Feb 26;9(3):. (PMID: 32110875)
Lancet. 2020 Feb 15;395(10223):507-513. (PMID: 32007143)
JAMA. 2020 May 12;323(18):1843-1844. (PMID: 32159775)
JAMA. 2020 Apr 21;323(15):1502-1503. (PMID: 32105304)
JAMA. 2020 Apr 7;323(13):1239-1242. (PMID: 32091533)
Nature. 2020 Mar;579(7798):270-273. (PMID: 32015507)
Lancet. 2020 Feb 15;395(10223):497-506. (PMID: 31986264)
Mil Med Res. 2020 Feb 6;7(1):4. (PMID: 32029004)
J Med Virol. 2020 May 15;:. (PMID: 32410245)
Grant Information:
2018BFG02008 International the Special Science and Technology Cooperation Project of Ningxia Hui Autonomous Region Key R&D Program; 2020BEG01004 International Special New Coronary Pneumonia Epidemic Prevention and Control Project of Ningxia Hui Autonomous Region
Contributed Indexing:
Keywords: COVID-19; Discharge criteria; Multiple specimens; RT-qPCR; SARS-CoV-2
Substance Nomenclature:
0 (RNA, Viral)
Entry Date(s):
Date Created: 20201103 Date Completed: 20201116 Latest Revision: 20201218
Update Code:
20240105
PubMed Central ID:
PMC7605325
DOI:
10.1186/s12967-020-02580-w
PMID:
33138834
Czasopismo naukowe
Background: The SARS-CoV-2 RNA was detected positive again after discharged from hospital in some COVID-19 patients, with or without clinical symptoms such as fever or dry cough.
Methods: 1008 severe COVID-19 patients, with SARS-CoV-2 RNA positive detected with the mixed specimen of nasopharyngeal swab and oropharyngeal swab by real-time fluorescence quantitative PCR (RT-qPCR), were selected to monitor SARS-CoV-2 RNA with the 12 types of specimens by RT-qPCR during hospitalization. All of 20 discharged cases with COVID-19 were selected to detect SARS-CoV-2 RNA in isolation period with 7 types of specimens by RT-qPCR before releasing the isolation period.
Results: Of the enrolled 1008 severe patients, the nasopharyngeal swab specimens showed the highest positive rate of SARS-CoV-2 RNA (71.06%), followed by alveolar lavage fluid (66.67%), oropharyngeal swab (30.77%), sputum (28.53%), urine (16.30%), blood (12.5%), stool (12.21%), anal swab (11.22%) and corneal secretion (2.99%), and SARS-CoV-2 RNA couldn't be detected in other types of specimen in this study. Of the 20 discharged cases during the isolation period, the positive rate of SARS-CoV-2 RNA was 30% (6/20): 2 cases were positive in sputum at the eighth and ninth day after discharge, respectively, 1 case was positive in nasopharynx swab at the sixth day after discharge, 1 case was positive in anal swab at the eighth day after discharge, and 1 case was positive in 3 specimens (nasopharynx swab, oropharynx swab and sputum) simultaneously at the fourth day after discharge, and no positive SARS-CoV-2 RNA was detected in other specimens including stool, urine and blood at the discharged patients.
Conclusions: SARS-CoV-2 RNA should be detected in multiple specimens, such as nasopharynx swab, oropharynx swab, sputum, and if necessary, stool and anal swab specimens should be performed simultaneously at discharge when the patients were considered for clinical cure and before releasing the isolation period.
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