Bioactive potential of endophytic fungus Chaetomium globosum and GC-MS analysis of its responsible components.

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Abstrakt

Tytuł:: Bioactive potential of endophytic fungus Chaetomium globosum and GC-MS analysis of its responsible components.
Autorzy:: Kaur N; Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India.
Arora DS; Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India. daljit_.
Kalia N; Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar, Punjab, 143005, India.
Kaur M; Department of Human Genetics, Guru Nanak Dev University, Amritsar, 143005, India.
Źródło:: Scientific reports [Sci Rep] 2020 Nov 02; Vol. 10 (1), pp. 18792. Date of Electronic Publication: 2020 Nov 02.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: London : Nature Publishing Group, copyright 2011-
MeSH Terms:: Antimutagenic Agents*
Chaetomium*/chemistry
Endophytes*
Cell Extracts/*pharmacology
Moringa oleifera/*microbiology
Antioxidants ; Cell Extracts/analysis ; Cell Extracts/isolation & purification ; Cell Line ; Cell Proliferation/drug effects ; Dose-Response Relationship, Drug ; Drug Development ; Flavonoids/analysis ; Flavonoids/isolation & purification ; Flavonoids/pharmacology ; Gas Chromatography-Mass Spectrometry ; Humans ; Phenols/analysis ; Phenols/isolation & purification ; Phenols/pharmacology
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Substance Nomenclature:: 0 (Antimutagenic Agents)
0 (Antioxidants)
0 (Cell Extracts)
0 (Flavonoids)
0 (Phenols)
SCR Organism:: Chaetomium globosum
Entry Date(s):: Date Created: 20201103 Date Completed: 20210406 Latest Revision: 20210406
Update Code:: 20240105
PubMed Central ID:: PMC7606472
DOI:: 10.1038/s41598-020-75722-1
PMID:: 33139805
: Czasopismo naukowe

Pełny tekst

The recent exploration of various medicinal plants for bioactive potential has led to the growing interest to explore their endophytes for such bioactive potential which may turn out to be better option than the plants. In the present study, Chaetomium globosum, an endophytic fungus isolated from Moringa oleifera Lam has been explored for its various biological activities. The chloroformic extract of C. globosum showed good antimutagenicity against the reactive carcinogenic mutagen, 2-aminofluorene (2-AF) in Ames test. The antiproliferative activity against various cell lines such as HCT-15, HeLa and U87-MG was found to be dose dependent and the viability reduced to 9.26%, 15.7% and 16.3%, respectively. Further, the chloroformic fungal extract was investigated for free radical scavenging activity using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethyl-benzthiazolin-6-sulfonic acid) assay which showed the IC 50 value of 45.16 µg/ml and 50.55 µg/ml, respectively. The fungal extract also showed good ferric reducing power. Total phenolic and flavonoid content was found to be in linear relationship with the antioxidant potential of the fungal extract. High performance liquid chromatography showed the presence of phenolics which may help to combat the free radicals. The presence of various bioactive compounds was analysed by GC-MS which endorsed Chaetomium globosum to be a promising candidate for drug development.

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