Doxycycline-Coated Silicone Breast Implants Reduce Acute Surgical-Site Infection and Inflammation.

Tytuł:: Doxycycline-Coated Silicone Breast Implants Reduce Acute Surgical-Site Infection and Inflammation.
Autorzy:: Baker JE; From the Sections of Surgical Research and Plastic, Reconstructive, and Hand Surgery, Department of Surgery, and the Department of Chemistry, College of Arts and Science, University of Cincinnati; the Division of Surgery, Shriners Hospital for Children; and the Department of Molecular Biology, University Hospital Essen, University of Duisburg-Essen.
Seitz AP; From the Sections of Surgical Research and Plastic, Reconstructive, and Hand Surgery, Department of Surgery, and the Department of Chemistry, College of Arts and Science, University of Cincinnati; the Division of Surgery, Shriners Hospital for Children; and the Department of Molecular Biology, University Hospital Essen, University of Duisburg-Essen.
Boudreau RM; From the Sections of Surgical Research and Plastic, Reconstructive, and Hand Surgery, Department of Surgery, and the Department of Chemistry, College of Arts and Science, University of Cincinnati; the Division of Surgery, Shriners Hospital for Children; and the Department of Molecular Biology, University Hospital Essen, University of Duisburg-Essen.
Skinner MJ; From the Sections of Surgical Research and Plastic, Reconstructive, and Hand Surgery, Department of Surgery, and the Department of Chemistry, College of Arts and Science, University of Cincinnati; the Division of Surgery, Shriners Hospital for Children; and the Department of Molecular Biology, University Hospital Essen, University of Duisburg-Essen.
Beydoun A; From the Sections of Surgical Research and Plastic, Reconstructive, and Hand Surgery, Department of Surgery, and the Department of Chemistry, College of Arts and Science, University of Cincinnati; the Division of Surgery, Shriners Hospital for Children; and the Department of Molecular Biology, University Hospital Essen, University of Duisburg-Essen.
Kaval N; From the Sections of Surgical Research and Plastic, Reconstructive, and Hand Surgery, Department of Surgery, and the Department of Chemistry, College of Arts and Science, University of Cincinnati; the Division of Surgery, Shriners Hospital for Children; and the Department of Molecular Biology, University Hospital Essen, University of Duisburg-Essen.
Caldwell CC; From the Sections of Surgical Research and Plastic, Reconstructive, and Hand Surgery, Department of Surgery, and the Department of Chemistry, College of Arts and Science, University of Cincinnati; the Division of Surgery, Shriners Hospital for Children; and the Department of Molecular Biology, University Hospital Essen, University of Duisburg-Essen.
Gulbins E; From the Sections of Surgical Research and Plastic, Reconstructive, and Hand Surgery, Department of Surgery, and the Department of Chemistry, College of Arts and Science, University of Cincinnati; the Division of Surgery, Shriners Hospital for Children; and the Department of Molecular Biology, University Hospital Essen, University of Duisburg-Essen.
Edwards MJ; From the Sections of Surgical Research and Plastic, Reconstructive, and Hand Surgery, Department of Surgery, and the Department of Chemistry, College of Arts and Science, University of Cincinnati; the Division of Surgery, Shriners Hospital for Children; and the Department of Molecular Biology, University Hospital Essen, University of Duisburg-Essen.
Gobble RM; From the Sections of Surgical Research and Plastic, Reconstructive, and Hand Surgery, Department of Surgery, and the Department of Chemistry, College of Arts and Science, University of Cincinnati; the Division of Surgery, Shriners Hospital for Children; and the Department of Molecular Biology, University Hospital Essen, University of Duisburg-Essen.
Źródło:: Plastic and reconstructive surgery [Plast Reconstr Surg] 2020 Nov; Vol. 146 (5), pp. 1029-1041.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: : Hagerstown, MD : Lippincott Williams & Wilkins
Original Publication: Baltimore : Williams & Wilkins,
MeSH Terms:: Breast Implants*
Methicillin-Resistant Staphylococcus aureus*
Prosthesis Design*
Pseudomonas aeruginosa*
Silicone Gels*
Anti-Bacterial Agents/*therapeutic use
Coated Materials, Biocompatible/*therapeutic use
Doxycycline/*therapeutic use
Mastitis/*prevention & control
Pseudomonas Infections/*prevention & control
Staphylococcal Infections/*prevention & control
Surgical Wound Infection/*prevention & control
Acute Disease ; Animals ; Male ; Mice ; Postoperative Complications/prevention & control
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Substance Nomenclature:: 0 (Anti-Bacterial Agents)
0 (Coated Materials, Biocompatible)
0 (Silicone Gels)
N12000U13O (Doxycycline)
Entry Date(s):: Date Created: 20201103 Date Completed: 20201231 Latest Revision: 20210106
Update Code:: 20240105
DOI:: 10.1097/PRS.0000000000007277
PMID:: 33141530
: Czasopismo naukowe

Background: Surgical-site infection after implant-based breast reconstruction remains a leading cause of morbidity. Doxycycline is an antibiotic used to treat soft-tissue infections. The authors hypothesize that doxycycline-coated breast implants will significantly reduce biofilm formation, surgical-site infection, and inflammation after bacterial infection.
Methods: Pieces of silicone breast implants were coated in doxycycline. In vitro studies to characterize the coating include Fourier transmission infrared spectroscopy, elution data, and toxicity assays (n = 4). To evaluate antimicrobial properties, coated implants were studied after methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa inoculation in vitro and in a mouse model at 3 and 7 days (n = 8). Studies included bacterial quantification, cytokine profiles, and histology.
Results: Coated silicone breast implants demonstrated a color change, increased mass, and Fourier transmission infrared spectroscopy consistent with a doxycycline coating. Coated implants were nontoxic to fibroblasts and inhibited biofilm formation and bacterial adherence after MRSA and P. aeruginosa incubation in vitro, and measurable doxycycline concentrations at 24 hours were seen. In a mouse model, a significant reduction of MRSA and P. aeruginosa bacterial colonization after 3 and 7 days in the doxycycline-coated implant mice was demonstrated when compared to the control mice, control mice treated with intraperitoneal doxycycline, and control mice treated with a gentamicin/cefazolin/bacitracin wash. Decreased inflammatory cytokines and inflammatory cell infiltration were demonstrated in the doxycycline-coated mice.
Conclusions: A method to coat silicone implants with doxycycline was developed. The authors' doxycycline-coated silicone implants significantly reduced biofilm formation, surgical-site infections, and inflammation. Further studies are needed to evaluate the long-term implications.

Comment in: Plast Reconstr Surg. 2020 Nov;146(5):1042-1043. (PMID: 33136950)

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