Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Tytuł pozycji:

Treatment with GDF15, a TGFβ superfamily protein, induces protective effect on retinal ganglion cells.

Tytuł:
Treatment with GDF15, a TGFβ superfamily protein, induces protective effect on retinal ganglion cells.
Autorzy:
Iwata Y; Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, Gifu, Japan.
Inagaki S; Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, Gifu, Japan.
Morozumi W; Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, Gifu, Japan.
Nakamura S; Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, Gifu, Japan.
Hara H; Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, Gifu, Japan.
Shimazawa M; Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, Gifu, Japan. Electronic address: .
Źródło:
Experimental eye research [Exp Eye Res] 2021 Jan; Vol. 202, pp. 108338. Date of Electronic Publication: 2020 Nov 03.
Typ publikacji:
Journal Article
Język:
English
Imprint Name(s):
Publication: London : Academic Press
Original Publication: London.
MeSH Terms:
Growth Differentiation Factor 15/*pharmacology
Optic Nerve Injuries/*prevention & control
Retinal Degeneration/*prevention & control
Retinal Ganglion Cells/*drug effects
Animals ; Blotting, Western ; Cells, Cultured ; Disease Models, Animal ; Glial Fibrillary Acidic Protein/metabolism ; Growth Differentiation Factor 15/metabolism ; Immunohistochemistry ; Male ; Mice ; Microscopy, Confocal ; Nerve Crush ; Optic Nerve Injuries/metabolism ; Rats ; Rats, Sprague-Dawley ; Retinal Degeneration/metabolism ; Retinal Ganglion Cells/metabolism ; Tissue Distribution ; Transforming Growth Factor beta/pharmacology
Substance Nomenclature:
0 (GFAP protein, rat)
0 (Glial Fibrillary Acidic Protein)
0 (Growth Differentiation Factor 15)
0 (Transforming Growth Factor beta)
Entry Date(s):
Date Created: 20201106 Date Completed: 20210416 Latest Revision: 20210416
Update Code:
20240105
DOI:
10.1016/j.exer.2020.108338
PMID:
33157126
Czasopismo naukowe
Growth differentiation factor 15 (GDF15) is a protein belonging to the transforming growth factor beta (TGF-β) superfamily. The precursor GDF15 is cleaved and activated as a mature GDF15 by protease. GDF15 has been detected in the aqueous humor of the primary open angle glaucoma patients, however the localization and the effect on the retinal ganglion cells (RGCs) are still unknown. Thus, the purpose of this study was to elucidate the effect of GDF15 on mouse optic nerve crush (ONC) model and primary culture of rat RGCs. Immunostaining showed that the GDF15 was in the ganglion cell layer (GCL), and colocalized with GFAP-positive cells in the GCL and the optic nerve. Western blotting analysis showed that the mature GDF15 was upregulated in the retina and the optic nerve after the ONC. Intravitreal injection of GDF15 suppressed RGCs loss of the ONC model mice in vivo. The neurites length of the primary culture of rat RGCs were increased by mature GDF15 treatment. In addition, the neurotrophic effect of GDF15 was canceled by RET inhibitor treatment. These findings indicate that GDF15 has neuroprotective effect on RGCs via GFRAL-RET pathway. Therefore, GDF15 may be one of novel therapeutic targets in RGC degenerative diseases.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies