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Tytuł:
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Cell Analysis on Microfluidics Combined with Mass Spectrometry.
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Autorzy:
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Zhang W; Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, 100084, China.
Zhang Q; Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, 100084, China.
Lin JM; Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, 100084, China. .
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Źródło:
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Analytical sciences : the international journal of the Japan Society for Analytical Chemistry [Anal Sci] 2021 Feb 10; Vol. 37 (2), pp. 249-260. Date of Electronic Publication: 2020 Nov 06.
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Typ publikacji:
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Journal Article; Review
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Język:
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English
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Imprint Name(s):
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Publication: 2022- : [Cham] : Springer
Original Publication: Tokyo : The Society, [1985-
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MeSH Terms:
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Tissue Array Analysis*
Cell Culture Techniques ; Humans ; Mass Spectrometry ; Microfluidic Analytical Techniques
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Contributed Indexing:
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Keywords: Microfluidic; cell analysis; cell metabolism; mass spectrometer; single-cell
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Entry Date(s):
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Date Created: 20201109 Date Completed: 20210927 Latest Revision: 20230808
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Update Code:
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20240105
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DOI:
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10.2116/analsci.20R006
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PMID:
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33162415
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Cell analysis is of great significance for the exploration of human diseases and health. However, there are not many techniques for high-throughput cell analysis in the simulated cell microenvironment. The high designability of the microfluidic chip enables multiple kinds of cells to be co-cultured on the chip, with other functions such as sample preprocessing and cell manipulation. Mass spectrometry (MS) can detect a large number of biomolecules without labelling. Therefore, the application of the microfluidic chip coupled with MS has represented a major branch of cell analysis over the past decades. Here, we concisely introduce various microfluidic devices coupled with MS used for cell analysis. The main functions of microfluidic devices are described first, followed by introductions of different interfaces with different types of MS. Then, their various applications in cell analysis are highlighted, with an emphasis on cell metabolism, drug screening, and signal transduction. Current limitations and prospective trends of microfluidics coupled with MS are discussed at the end.