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Tytuł pozycji:

Hyaluronan alkyl derivatives-based electrospun membranes for potential guided bone regeneration: Fabrication, characterization and in vitro osteoinductive properties.

Tytuł :
Hyaluronan alkyl derivatives-based electrospun membranes for potential guided bone regeneration: Fabrication, characterization and in vitro osteoinductive properties.
Autorzy :
Federico S; Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF), Università degli Studi di Palermo, Via Archirafi 32, 90123, Palermo, Italy.
Pitarresi G; Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF), Università degli Studi di Palermo, Via Archirafi 32, 90123, Palermo, Italy.
Palumbo FS; Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF), Università degli Studi di Palermo, Via Archirafi 32, 90123, Palermo, Italy. Electronic address: .
Fiorica C; Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF), Università degli Studi di Palermo, Via Archirafi 32, 90123, Palermo, Italy.
Yang F; Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, Department of Dentistry - Regenerative Biomaterials, Philips van Leydenlaan 25, Nijmegen, the Netherlands.
Giammona G; Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche (STEBICEF), Università degli Studi di Palermo, Via Archirafi 32, 90123, Palermo, Italy; Institute of Biophysics at Palermo, Italian National Research Council, Via Ugo La Malfa 153, 90146, Palermo, Italy.
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Źródło :
Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2021 Jan; Vol. 197, pp. 111438. Date of Electronic Publication: 2020 Nov 04.
Typ publikacji :
Journal Article
Język :
English
Imprint Name(s) :
Original Publication: Amsterdam ; New York : Elsevier, c1993-
Contributed Indexing :
Keywords: Dexamethasone; Electrospun nanofibers; GBR membranes; Hyaluronic acid alkylated
Entry Date(s) :
Date Created: 20201109 Latest Revision: 20201215
Update Code :
20201231
DOI :
10.1016/j.colsurfb.2020.111438
PMID :
33166935
Czasopismo naukowe
The aim of the work was to determine the effects of the chemical functionalization of hyaluronic acid (HA) with pendant aliphatic tails at different lengths and free amino groups in terms of chemical reactivity, degradation rate, drug-eluting features, and surface properties when processed as electrospun membranes (EM) evaluating the osteoinductive potential for a possible application as guided bone regeneration (GBR). To this end, a series of HA derivatives with different aliphatic tails (DD-Cx mol% ≈ 12.0 mol%) and decreasing derivatization of free amino groups (DD EDA mol% from 70.0 to 30.0 mol%) were first synthesized, namely Hn. Then dexamethasone-loaded Hn EM, i.e. HnX were prepared from aqueous polymeric solutions with polyvinyl alcohol (PVA), as a non-ionogenic linear flexible polymeric carrier, and the multifunctional 2-hydroxypropyl- cyclodextrin (HPCD) which acted as a rheological modifier, a stabilizer of Taylor's cone, and a solubilizing agent. A comprehensive characterization of the membranes was carried out through ATR-IR, XRD, and WCA measurements. According to the in vitro hydrolytic and enzymatic degradation and drug release in different aqueous media for two months, the insertion of alkyl pendant grafts and the crosslinking process provided tuneable additional resistance to the whole membrane suitably for the final application of the membranes. Cell culture showed the cytocompatibility and cell proliferation until 7 days. Osteogenic differentiation and mineralization of pre-osteoblastic MC3T3 cells occurred for most of membranes after 35 days as valued by measuring ALP activity (50 nmol 4-np/h/nf DNA) and the deposition of calcium (120-140 μg ml -1 ).
(Copyright © 2020 Elsevier B.V. All rights reserved.)

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