Generation of paternal haploids in wheat by genome editing of the centromeric histone CENH3.

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Abstrakt

Tytuł:: Generation of paternal haploids in wheat by genome editing of the centromeric histone CENH3.
Autorzy:: Lv J; Seeds Research, Syngenta Biotechnology China, ZhongGuanCun Life Science Park, Beijing, China. .
Yu K; Seeds Research, Syngenta Biotechnology China, ZhongGuanCun Life Science Park, Beijing, China.
Wei J; Seeds Research, Syngenta Biotechnology China, ZhongGuanCun Life Science Park, Beijing, China.
Gui H; Seeds Research, Syngenta Biotechnology China, ZhongGuanCun Life Science Park, Beijing, China.
Liu C; Seeds Research, Syngenta Biotechnology China, ZhongGuanCun Life Science Park, Beijing, China.
Liang D; Seeds Research, Syngenta Biotechnology China, ZhongGuanCun Life Science Park, Beijing, China.
Wang Y; Seeds Research, Syngenta Biotechnology China, ZhongGuanCun Life Science Park, Beijing, China.
Zhou H; Seeds Research, Syngenta Biotechnology China, ZhongGuanCun Life Science Park, Beijing, China.
Carlin R; Seeds Research, Syngenta Crop Protection, LLC, Research Triangle Park, NC, USA.
Rich R; Seeds Research, Syngenta Seeds Research, Junction City, KS, USA.
Lu T; Seeds Research, Syngenta Biotechnology China, ZhongGuanCun Life Science Park, Beijing, China.
Que Q; Seeds Research, Syngenta Crop Protection, LLC, Research Triangle Park, NC, USA.
Wang WC; Seeds Research, Syngenta Crop Protection, LLC, Research Triangle Park, NC, USA.
Zhang X; Seeds Research, Syngenta Biotechnology China, ZhongGuanCun Life Science Park, Beijing, China.
Kelliher T; Seeds Research, Syngenta Crop Protection, LLC, Research Triangle Park, NC, USA. .
Źródło:: Nature biotechnology [Nat Biotechnol] 2020 Dec; Vol. 38 (12), pp. 1397-1401. Date of Electronic Publication: 2020 Nov 09.
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Publication: New York Ny : Nature America Publishing
Original Publication: New York, NY : Nature Pub. Co., [1996-
MeSH Terms:: Gene Editing*
Haploidy*
Centromere/*metabolism
Histones/*metabolism
Triticum/*genetics
Alleles ; Amino Acid Sequence ; Base Sequence ; Crosses, Genetic ; Diploidy ; Histones/chemistry ; Phenotype
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Substance Nomenclature:: 0 (Histones)
Entry Date(s):: Date Created: 20201110 Date Completed: 20210120 Latest Revision: 20210629
Update Code:: 20240105
DOI:: 10.1038/s41587-020-0728-4
PMID:: 33169035
: Czasopismo naukowe

New breeding technologies accelerate germplasm improvement and reduce the cost of goods in seed production 1-3 . Many such technologies could use in vivo paternal haploid induction (HI), which occurs when double fertilization precedes maternal (egg cell) genome loss. Engineering of the essential CENTROMERIC HISTONE (CENH3) gene induces paternal HI in Arabidopsis 4-6 . Despite conservation of CENH3 function across crops, CENH3-based HI has not been successful outside of the Arabidopsis model system 7 . Here we report a commercially operable paternal HI line in wheat with a ~7% HI rate, identified by screening genome-edited TaCENH3α-heteroallelic combinations. Unlike in Arabidopsis, edited alleles exhibited reduced transmission in female gametophytes, and heterozygous genotypes triggered higher HI rates than homozygous combinations. These developments might pave the way for the deployment of CENH3 HI technology in diverse crops.

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