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Tytuł:
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A simple and rapid immunochromatography test based on readily available filter paper modified with chitosan to screen for 13 sulfonamides in milk.
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Autorzy:
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Zeng Y; Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, 100193 Beijing, People's Republic of China.
Liang D; Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, 100193 Beijing, People's Republic of China.
Zheng P; Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, 100193 Beijing, People's Republic of China.
Zhang Y; Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, 100193 Beijing, People's Republic of China.
Wang Z; Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, 100193 Beijing, People's Republic of China.
Mari GM; Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, 100193 Beijing, People's Republic of China.
Jiang H; Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, 100193 Beijing, People's Republic of China. Electronic address: .
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Źródło:
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Journal of dairy science [J Dairy Sci] 2021 Jan; Vol. 104 (1), pp. 126-133. Date of Electronic Publication: 2020 Nov 12.
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Typ publikacji:
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Journal Article; Validation Study
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Język:
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English
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Imprint Name(s):
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Publication: Champaign, IL : American Dairy Science Association
Original Publication: Lancaster, Pa. [etc.]
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MeSH Terms:
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Chitosan/*chemistry
Chromatography, Affinity/*veterinary
Milk/*chemistry
Sulfonamides/*analysis
Animals ; Chromatography, Affinity/instrumentation ; Chromatography, Affinity/methods ; Filtration/instrumentation ; Gold Colloid/analysis ; Gold Colloid/chemistry ; Paper ; Vaccines
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Contributed Indexing:
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Keywords: chitosan; filter paper; lateral flow device; sulfonamides
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Substance Nomenclature:
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0 (Gold Colloid)
0 (Sulfonamides)
0 (Vaccines)
9012-76-4 (Chitosan)
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Entry Date(s):
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Date Created: 20201115 Date Completed: 20210222 Latest Revision: 20210222
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Update Code:
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20240105
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DOI:
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10.3168/jds.2020-18987
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PMID:
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33189293
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In this study, we developed a novel, simple, rapid, and low-cost colloidal gold-based immunochromatography method, with filter paper replacing nitrocellulose membrane as the substrate. To obtain adequately immobilized protein, chitosan was used to functionalize the filter paper. After conditions and parameters were optimized, the novel immunochromatography method was applied for detection of sulfonamide residues in milk. Quantitative detection was accomplished using a smartphone and Photoshop software (Adobe Inc., San Jose, CA), allowing us to screen 13 sulfonamides with a limit of detection ranging from 0.42 to 8.64 μg/L and recovery ranging from 88.2 to 116.9% in milk. The proposed novel method performed similarly to the conventional method that uses a nitrocellulose membrane as the transport medium, and it had lower cost and better usability because of the inexpensive and easily available filter paper.
(Copyright © 2021 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)
