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Tytuł pozycji:

Ceramide ratios are affected by cigarette smoke but not heat-not-burn or e-vapor aerosols across four independent mouse studies.

Tytuł :
Ceramide ratios are affected by cigarette smoke but not heat-not-burn or e-vapor aerosols across four independent mouse studies.
Autorzy :
Lavrynenko O; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland. Electronic address: .
Titz B; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
Dijon S; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
Santos DD; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
Nury C; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
Schneider T; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
Guedj E; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
Szostak J; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
Kondylis A; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
Phillips B; Philip Morris International Research Laboratories Pte. Ltd., Science Park II, 117406, Singapore.
Ekroos K; Lipidomics Consulting Ltd., Irisviksvägen 31D, 02230 Esbo, Finland.
Martin F; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
Peitsch MC; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
Hoeng J; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
Ivanov NV; PMI R&D, Philip Morris Products S.A., Quai Jeanrenaud 5, CH-2000 Neuchâtel, Switzerland.
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Źródło :
Life sciences [Life Sci] 2020 Dec 15; Vol. 263, pp. 118753. Date of Electronic Publication: 2020 Nov 12.
Typ publikacji :
Comparative Study; Journal Article; Validation Study
Język :
English
Imprint Name(s) :
Publication: <2008->: Amsterdam : Elsevier
Original Publication: Oxford; Elmsford, N. Y. [etc.] Pergamon Press.
MeSH Terms :
Apolipoproteins E/*genetics
Ceramides/*metabolism
E-Cigarette Vapor/*adverse effects
Lung/*metabolism
Smoke/*adverse effects
Aerosols/adverse effects ; Animals ; Ceramides/analysis ; Chromatography, Liquid/methods ; Female ; Humans ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Proteomics ; Risk Factors ; Tandem Mass Spectrometry/methods ; Time Factors
Contributed Indexing :
Keywords: COPD; Ceramides; Cigarette smoke; E-cigarettes; Heat-not-burn tobacco product; Lipidomics
Substance Nomenclature :
0 (Aerosols)
0 (Apolipoproteins E)
0 (Ceramides)
0 (E-Cigarette Vapor)
0 (Smoke)
Entry Date(s) :
Date Created: 20201115 Date Completed: 20201215 Latest Revision: 20201215
Update Code :
20201231
DOI :
10.1016/j.lfs.2020.118753
PMID :
33189821
Czasopismo naukowe
Aims: Smoking is an important risk factor for the development of chronic obstructive pulmonary disease and cardiovascular diseases. This study aimed to further elucidate the role of ceramides, as a key lipid class dysregulated in disease states.
Main Methods: In this article we developed and validated LC-MS/MS method for ceramides (Cer(d18:1/16:0), Cer(d18:1/18:0), Cer(d18:1/24:0) and Cer(d18:1/24:1(15Z)) for the absolute quantification. We deployed it together with proteomics and transcriptomic analysis to assess the effects of cigarette smoke (CS) from the reference cigarette as well as aerosols from heat-not-burn (HnB) tobacco and e-vapor products in apolipoprotein E-deficient (ApoE -/- ) mice over several time points.
Key Findings: In the lungs, CS exposure substantially elevated the ratios of Cer(d18:1/24:0) and Cer(d18:1/24:1) to Cer(d18:1/18:0) in two independent ApoE -/- mouse inhalation studies. Data from previous studies, in both ApoE -/- and wild-type mice, further confirmed the reproducibility of this finding. Elevation of these ceramide ratios was also observed in plasma/serum, the liver, and-for the Cer(d18:1/24:1(15Z)) to Cer(d18:1/18:0) ratio-the abdominal aorta. Also, the levels of acid ceramidase (Asah1) and glucocerebrosidase (Gba)-lysosomal enzymes involved in the hydrolysis of glucosylceramides-were consistently elevated in the lungs after CS exposure. In contrast, exposure to HnB tobacco product and e-vapor aerosols did not induce significant changes in the ceramide profiles or associated enzymes.
Significance: Our work in mice contributes to the accumulating evidence on the importance of ceramide ratios as biologically relevant markers for respiratory disorders, adding to their already demonstrated role in cardiovascular disease risk assessment in humans.
(Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)

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