A Mouse Femoral Ostectomy Model to Assess Bone Graft Substitutes.

Szczegóły
Abstrakt

Tytuł:: A Mouse Femoral Ostectomy Model to Assess Bone Graft Substitutes.
Autorzy:: Trombetta RP; Orthopedic Trauma Department, US Army Institute for Surgical Research, San Antonio, TX, USA.
Knapp EK; Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY, USA.
Awad HA; Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY, USA. Hani_.; Department of Biomedical Engineering, University of Rochester, Rochester, NY, USA. Hani_.
Źródło:: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2230, pp. 75-89.
Typ publikacji:: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Publication: Totowa, NJ : Humana Press
Original Publication: Clifton, N.J. : Humana Press,
MeSH Terms:: Biocompatible Materials/*pharmacology
Bone Regeneration/*drug effects
Bone Transplantation/*methods
Diaphyses/*drug effects
Femur/*surgery
Animals ; Autografts/transplantation ; Bone Screws ; Bone Substitutes/pharmacology ; Diaphyses/growth & development ; Disease Models, Animal ; Femur/growth & development ; Femur/physiopathology ; Fracture Healing/drug effects ; Humans ; Mice
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Grant Information:: P30 AR061307 United States AR NIAMS NIH HHS; P30 AR069655 United States AR NIAMS NIH HHS; P50 AR072000 United States AR NIAMS NIH HHS
Contributed Indexing:: Keywords: Biomechanics; Bone; Bone graft substitute; Critical sized defect; Healing; Mouse model; Scaffold; Torsional testing
Substance Nomenclature:: 0 (Biocompatible Materials)
0 (Bone Substitutes)
Entry Date(s):: Date Created: 20201116 Date Completed: 20210324 Latest Revision: 20240402
Update Code:: 20240402
PubMed Central ID:: PMC8299532
DOI:: 10.1007/978-1-0716-1028-2_5
PMID:: 33197009
: Czasopismo naukowe

The shortcomings of autografts and allografts in bone defect healing have prompted researchers to develop suitable alternatives. Numerous biomaterials have been developed as bone graft substitutes each with their own advantages and disadvantages. However, in order to test if these biomaterials provide an adequate replacement of the clinical standard, a clinically representative animal model is needed to test their efficacy. In this chapter, we describe a mouse model that establishes a critical sized defect in the mid-diaphysis of the femur to evaluate the performance of bone graft substitutes. This is achieved by performing a femoral ostectomy and stabilization utilizing a femoral plate and titanium screws. The resulting defect enables the bone regenerative potential of bone graft substitutes to be investigated. Lastly, we provide instruction on assessing the torsional strength of the healed femurs to quantitatively evaluate the degree of healing as a primary outcome measure.

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