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Tytuł pozycji:

Development of a recombinase polymerase amplification fluorescence assay to detect feline coronavirus.

Tytuł :
Development of a recombinase polymerase amplification fluorescence assay to detect feline coronavirus.
Autorzy :
Hu X; School of Life Sciences and Food Engineering, Yibin University, Yibin Key Laboratory of Zoological Diversity and Ecological Conservation, Yibin, 644000, China. Electronic address: .
Xiao L; Guangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou, 510633, China. Electronic address: .
Cong X; Anhui Science and Technology University, Chuzhou, 233100, China. Electronic address: .
Zhu Y; Guangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou, 510633, China. Electronic address: .
Huang B; Guangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou, 510633, China. Electronic address: .
Cong F; Guangdong Laboratory Animals Monitoring Institute and Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou, 510633, China. Electronic address: .
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Źródło :
Molecular and cellular probes [Mol Cell Probes] 2020 Dec; Vol. 54, pp. 101669. Date of Electronic Publication: 2020 Oct 22.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
Język :
English
Imprint Name(s) :
Original Publication: London ; New York : Academic Press, c1987-
MeSH Terms :
Coronavirus Infections/*diagnosis
Coronavirus, Feline/*genetics
Feline Infectious Peritonitis/*diagnosis
Molecular Diagnostic Techniques/*veterinary
Nucleic Acid Amplification Techniques/*methods
RNA, Viral/*genetics
Animals ; Cat Diseases/diagnosis ; Cat Diseases/virology ; Cats ; Coronavirus, Feline/isolation & purification ; Molecular Diagnostic Techniques/methods ; Nucleic Acid Amplification Techniques/veterinary ; Real-Time Polymerase Chain Reaction/methods ; Sensitivity and Specificity
References :
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Contributed Indexing :
Keywords: Detection*; Feline coronavirus*; Recombinase polymerase amplification*
Substance Nomenclature :
0 (RNA, Viral)
SCR Protocol :
LAMP assay
Entry Date(s) :
Date Created: 20201118 Date Completed: 20201223 Latest Revision: 20201223
Update Code :
20201231
PubMed Central ID :
PMC7581357
DOI :
10.1016/j.mcp.2020.101669
PMID :
33203619
Czasopismo naukowe
Feline coronavirus (FCoV) is classified into two pathotypes: the avirulent feline enteric coronavirus (FECV), and the virulent feline infectious peritonitis virus (FIPV). Rapid pathogen detection, which is efficient and convenient, is the best approach for early confirmatory diagnosis. In this study, we first developed and evaluated a rapid recombinase polymerase amplification (RPA) detection method for FCoV that can detect FCoV within 15 min at 39 °C. The detection limit of that assay was 233 copies/μL DNA molecules per reaction. The specificity was high: it did not cross-react with canine distemper virus (CDV), canine coronavirus (CCoV), canine adenovirus (CAV), feline calicivirus (FCV), feline herpesvirus (FHV), or feline parvovirus (FPV). This assay was evaluated using 42 clinical samples (30 diarrhea samples and 12 ascites samples). The coincidence rate between FCoV-RPA and RT-qPCR for detection in clinical samples was 95.2%. In summary, FCoV-RPA analysis provides an efficient, rapid, and sensitive detection method for FCoV.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)

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