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Tytuł:
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Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation.
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Autorzy:
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Chen R; Key Laboratory of Elemene Class Anti-cancer Chinese Medicine of Zhejiang Province, Engineering Laboratory of Development and Application of Traditional Chinese Medicine from Zhejiang Province, Holistic Integrative Pharmacy Institutes, School of Medicine, Hangzhou Normal University, Hangzhou, P. R. China.; Faculty of Preventive Medicine of Medical College, Hangzhou Normal University, Hangzhou, P. R. China.
Wei Q; Key Laboratory of Elemene Class Anti-cancer Chinese Medicine of Zhejiang Province, Engineering Laboratory of Development and Application of Traditional Chinese Medicine from Zhejiang Province, Holistic Integrative Pharmacy Institutes, School of Medicine, Hangzhou Normal University, Hangzhou, P. R. China.
Wei X; Key Laboratory of Elemene Class Anti-cancer Chinese Medicine of Zhejiang Province, Engineering Laboratory of Development and Application of Traditional Chinese Medicine from Zhejiang Province, Holistic Integrative Pharmacy Institutes, School of Medicine, Hangzhou Normal University, Hangzhou, P. R. China.
Liu Y; Key Laboratory of Elemene Class Anti-cancer Chinese Medicine of Zhejiang Province, Engineering Laboratory of Development and Application of Traditional Chinese Medicine from Zhejiang Province, Holistic Integrative Pharmacy Institutes, School of Medicine, Hangzhou Normal University, Hangzhou, P. R. China.
Zhang X; Key Laboratory of Elemene Class Anti-cancer Chinese Medicine of Zhejiang Province, Engineering Laboratory of Development and Application of Traditional Chinese Medicine from Zhejiang Province, Holistic Integrative Pharmacy Institutes, School of Medicine, Hangzhou Normal University, Hangzhou, P. R. China.
Chen X; Key Laboratory of Elemene Class Anti-cancer Chinese Medicine of Zhejiang Province, Engineering Laboratory of Development and Application of Traditional Chinese Medicine from Zhejiang Province, Holistic Integrative Pharmacy Institutes, School of Medicine, Hangzhou Normal University, Hangzhou, P. R. China.
Yin X; Key Laboratory of Elemene Class Anti-cancer Chinese Medicine of Zhejiang Province, Engineering Laboratory of Development and Application of Traditional Chinese Medicine from Zhejiang Province, Holistic Integrative Pharmacy Institutes, School of Medicine, Hangzhou Normal University, Hangzhou, P. R. China.
Xie T; Key Laboratory of Elemene Class Anti-cancer Chinese Medicine of Zhejiang Province, Engineering Laboratory of Development and Application of Traditional Chinese Medicine from Zhejiang Province, Holistic Integrative Pharmacy Institutes, School of Medicine, Hangzhou Normal University, Hangzhou, P. R. China.
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Źródło:
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PloS one [PLoS One] 2020 Nov 18; Vol. 15 (11), pp. e0242564. Date of Electronic Publication: 2020 Nov 18 (Print Publication: 2020).
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Typ publikacji:
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Journal Article; Research Support, Non-U.S. Gov't
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Język:
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English
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Imprint Name(s):
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Original Publication: San Francisco, CA : Public Library of Science
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MeSH Terms:
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Aldo-Keto Reductases/*metabolism
Glucose 1-Dehydrogenase/*metabolism
Hydroxybutyrates/*chemical synthesis
Biocatalysis ; Butyrates ; Catalysis ; Coenzymes/metabolism ; Enzymes, Immobilized/metabolism ; Hydroxybutyrates/chemistry ; Microwaves ; NADP/metabolism ; Oxidation-Reduction ; Temperature
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References:
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J Biosci Bioeng. 2008 Sep;106(3):286-91. (PMID: 18930007)
J Am Chem Soc. 2008 Aug 6;130(31):10048-9. (PMID: 18613673)
Int J Mol Sci. 2013 Jan 10;14(1):1232-77. (PMID: 23306150)
Anal Biochem. 1976 May 7;72:248-54. (PMID: 942051)
Appl Microbiol Biotechnol. 1997 Dec;48(6):699-703. (PMID: 9457798)
J Ind Microbiol Biotechnol. 2014 Nov;41(11):1609-16. (PMID: 25189809)
J Chromatogr A. 2007 Sep 7;1163(1-2):157-68. (PMID: 17631301)
Methods Mol Biol. 2020;2100:27-63. (PMID: 31939114)
Int J Biol Macromol. 2020 Feb 1;144:1013-1021. (PMID: 31669469)
Biotechnol Lett. 2007 Sep;29(9):1413-6. (PMID: 17541760)
J Ind Microbiol Biotechnol. 2018 Feb;45(2):111-121. (PMID: 29322283)
Biotechniques. 2004 Nov;37(5):790-6, 798-802. (PMID: 15560135)
Int J Biol Macromol. 2018 Mar;108:893-901. (PMID: 29102791)
Bioresour Technol. 2012 Jul;115:158-63. (PMID: 22153596)
J Ind Microbiol Biotechnol. 2012 Apr;39(4):529-36. (PMID: 22120648)
J Org Chem. 2009 Aug 21;74(16):6157-62. (PMID: 19601570)
Enzyme Microb Technol. 2000 Aug 1;27(3-5):319-324. (PMID: 10899559)
J Biosci Bioeng. 2009 Mar;107(3):219-24. (PMID: 19269581)
Bioresour Technol. 2011 Jan;102(2):469-74. (PMID: 20843684)
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Substance Nomenclature:
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0 (4-chloro-3-hydroxybutyric acid)
0 (Butyrates)
0 (Coenzymes)
0 (Enzymes, Immobilized)
0 (Hydroxybutyrates)
53-59-8 (NADP)
EC 1.1.1.- (Aldo-Keto Reductases)
EC 1.1.1.47 (Glucose 1-Dehydrogenase)
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Entry Date(s):
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Date Created: 20201118 Date Completed: 20210104 Latest Revision: 20231112
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Update Code:
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20240105
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PubMed Central ID:
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PMC7673530
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DOI:
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10.1371/journal.pone.0242564
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PMID:
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33206717
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One of the challenges in biocatalysis is the development of stable and efficient bi-enzymatic cascades for bio-redox reactions coupled to the recycling of soluble cofactors. Aldo-keto reductase (LEK) and glucose dehydrogenase (GDH) can be utilized as the NADPH recycling system for economic and efficient biocatalysis of (R)-4-chloro-3-hydroxybutanoate ((R)-CHBE), an important chiral pharmaceutical intermediate. The LEK and GDH was efficiently co-immobilized in mesocellular siliceous foams (MCFs) under microwave irradiation (CoLG-MIA). while they were also co-immobilized by entrapment in calcium alginate without MIA as control (CoLG-CA). The relative activity of CoLG-MIA was increased to 140% compared with that of free LEK. The CoLG-MIA exhibited a wider range of pH and temperature stabilities compared with other preparations. The thermal, storage and batch operational stabilities of microwave-assisted immobilized LEK-GDH were also improved. The NADPH recycling system exhibited the potential as the stable and efficient catalyst for the industrial preparation of (R)-CHBE.
Competing Interests: The authors have declared that no competing interests exist.
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