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Tytuł pozycji:

From in vitro towards in situ: structure-based investigation of ABC exporters by electron paramagnetic resonance spectroscopy.

Tytuł:
From in vitro towards in situ: structure-based investigation of ABC exporters by electron paramagnetic resonance spectroscopy.
Autorzy:
Bordignon E; Faculty of Chemistry and Biochemistry, Ruhr University Bochum, Bochum, Germany.
Seeger MA; Institute of Medical Microbiology, University of Zurich, Switzerland.
Galazzo L; Faculty of Chemistry and Biochemistry, Ruhr University Bochum, Bochum, Germany.
Meier G; Institute of Medical Microbiology, University of Zurich, Switzerland.
Źródło:
FEBS letters [FEBS Lett] 2020 Dec; Vol. 594 (23), pp. 3839-3856. Date of Electronic Publication: 2020 Dec 01.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't; Review
Język:
English
Imprint Name(s):
Publication: Jan. 2016- : West Sussex : John Wiley & Sons Ltd.
Original Publication: Amsterdam, North-Holland on behalf of the Federation of European Biochemical Societies.
MeSH Terms:
Electron Spin Resonance Spectroscopy*
ATP-Binding Cassette Transporters/*chemistry
ATP-Binding Cassette Transporters/*metabolism
Animals ; Humans ; In Vitro Techniques ; Structure-Activity Relationship
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Contributed Indexing:
Keywords: ABC transporters; DEER; EPR; MTSL; detergent; inside-out vesicles; liposomes; nanobody; spin label; sybody
Substance Nomenclature:
0 (ATP-Binding Cassette Transporters)
Entry Date(s):
Date Created: 20201121 Date Completed: 20210604 Latest Revision: 20210604
Update Code:
20240105
DOI:
10.1002/1873-3468.14004
PMID:
33219535
Czasopismo naukowe
ATP-binding cassette (ABC) exporters have been studied now for more than four decades, and recent structural investigation has produced a large number of protein database entries. Yet, important questions about how ABC exporters function at the molecular level remain debated, such as which are the molecular recognition hotspots and the allosteric couplings dynamically regulating the communication between the catalytic cycle and the export of substrates. This conundrum mainly arises from technical limitations confining all research to in vitro analysis of ABC transporters in detergent solutions or embedded in membrane-mimicking environments. Therefore, a largely unanswered question is how ABC exporters operate in situ, namely in the native membrane context of a metabolically active cell. This review focuses on novel mechanistic insights into type I ABC exporters gained through a unique combination of structure determination, biochemical characterization, generation of conformation-specific nanobodies/sybodies and double electron-electron resonance.
(© 2020 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.)

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