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Tytuł:
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Anti-inflammatory effects of vinpocetine in LPS-stimulated microglia via activation of AMPK.
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Autorzy:
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Zhou Q; Department of Pharmacy, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Suzhou, Jiangsu, 215002, P.R. China.
Guo D; Department of pharmacy, The Affiliated Suzhou Science and Technology Town Hospital of Nanjing Medical University, Suzhou, Jiangsu, 215153, P.R. China.; Jiangsu Key Laboratory of Neuropsychiatric Diseases Research and College of Pharmaceutical Sciences, Soochow University, Suzhou, Jiangsu, 215021, P.R. China.
Li X; Department of Pharmacy, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Suzhou, Jiangsu, 215002, P.R. China.
Wang Y; Department of Pharmacy, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Suzhou, Jiangsu, 215002, P.R. China.
Ye X; Department of Pharmacy, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Suzhou, Jiangsu, 215002, P.R. China.
Xue S; Department of Pharmacy, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Suzhou, Jiangsu, 215002, P.R. China.
Wang X; Department of Pharmacy, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Suzhou, Jiangsu, 215002, P.R. China.
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Źródło:
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Anais da Academia Brasileira de Ciencias [An Acad Bras Cienc] 2020 Nov 20; Vol. 92 (4), pp. e20200241. Date of Electronic Publication: 2020 Nov 20 (Print Publication: 2020).
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Typ publikacji:
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Journal Article
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Język:
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English
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Imprint Name(s):
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Original Publication: Rio De Janeiro : Academia Brasileira De Ciencias
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MeSH Terms:
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Lipopolysaccharides*/pharmacology
Microglia*
AMP-Activated Protein Kinases ; Anti-Inflammatory Agents/pharmacology ; Cyclooxygenase 2 ; Nitric Oxide ; Vinca Alkaloids
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Substance Nomenclature:
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0 (Anti-Inflammatory Agents)
0 (Lipopolysaccharides)
0 (Vinca Alkaloids)
31C4KY9ESH (Nitric Oxide)
543512OBTC (vinpocetine)
EC 1.14.99.1 (Cyclooxygenase 2)
EC 2.7.11.31 (AMP-Activated Protein Kinases)
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Entry Date(s):
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Date Created: 20201125 Date Completed: 20201127 Latest Revision: 20201127
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Update Code:
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20240105
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DOI:
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10.1590/0001-3765202020200241
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PMID:
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33237143
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Microglia are the resident immune cells in the central nervous system (CNS), which play important roles in the repair of neuroinflammatory injury. The present study investigated the anti-neuroinflammatory effects of vinpocetine induced by lipopolysaccharide (LPS) in BV2 microglia. BV2 microglia were pretreated with vinpocetine, and then stimulated with LPS (100 ng/mL). The cytotoxicity of BV2 microglia was assessed by MTT assay. The expression levels of nitrite oxide were measured by Griess assay. Proinflammatory cytokines and mediators were determined by Western blot, ELISA, or quantitative real-time PCR. Vinpocetine significantly decreased the generation of nitric oxide-inducible nitric oxide synthase (iNOS), cyclooxygenase- (COX-) 2 in a dose-dependent manner. In addition, vinpocetine decreased the production of pro-inflammatory cytokines such as tumor necrosis factor alpha (TNF-α), interleukin (IL)-6 and IL-1β. Furthermore, it was observed that phosphorylation levels of AMPK (Thr-172) decreased in LPS-stimulated BV2 microglia. Vinpocetine treatment increased AMPK phosphorylation in LPS-stimulated BV2 microglia. AMPK inhibition by siRNA blocked the anti-inflammatory effects of vinpocetine induced by LPS in BV2 microglia. The overall results demonstrate that vinpocetine has anti-inflammatory effects on LPS-stimulated BV2 microglia via inducing phosphorylation of AMPK, suggesting that vinpocetine is a potential therapeutic agent in neuroinflammatory injury.
