Neuroprotective Effects of Curcumin-Loaded Emulsomes in a Laser Axotomy-Induced CNS Injury Model.

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Abstrakt

Tytuł:: Neuroprotective Effects of Curcumin-Loaded Emulsomes in a Laser Axotomy-Induced CNS Injury Model.
Autorzy:: Yilmaz EN; Regenerative and Restorative Medicine Research Center (REMER), Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, Beykoz, Istanbul, Turkey.; Graduate School of Engineering and Natural Sciences, Istanbul Medipol University, Beykoz, Istanbul, Turkey.
Bay S; Regenerative and Restorative Medicine Research Center (REMER), Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, Beykoz, Istanbul, Turkey.
Ozturk G; Regenerative and Restorative Medicine Research Center (REMER), Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, Beykoz, Istanbul, Turkey.; Department of Physiology, International School of Medicine, Istanbul Medipol University, Beykoz, Istanbul, Turkey.
Ucisik MH; Regenerative and Restorative Medicine Research Center (REMER), Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, Beykoz, Istanbul, Turkey.; Department of Biomedical Engineering, School of Engineering and Natural Sciences, Istanbul Medipol University, Beykoz, Istanbul, Turkey.
Źródło:: International journal of nanomedicine [Int J Nanomedicine] 2020 Nov 20; Vol. 15, pp. 9211-9229. Date of Electronic Publication: 2020 Nov 20 (Print Publication: 2020).
Typ publikacji:: Journal Article
Język:: English
Imprint Name(s):: Original Publication: Auckland : DOVE Medical Press,
MeSH Terms:: Curcumin/*pharmacology
Drug Delivery Systems/*methods
Emulsions/*chemistry
Neurons/*drug effects
Neuroprotective Agents/*pharmacology
Animals ; Antioxidants/pharmacology ; Axotomy ; Biomarkers/metabolism ; Cell Survival/drug effects ; Cells, Cultured ; Central Nervous System/injuries ; Curcumin/administration & dosage ; Curcumin/pharmacokinetics ; Emulsions/administration & dosage ; Hippocampus/cytology ; Hippocampus/drug effects ; Lasers ; Mice, Inbred BALB C ; Nanostructures/administration & dosage ; Nanostructures/chemistry ; Neurons/cytology ; Neurons/metabolism ; Neuroprotective Agents/administration & dosage ; Neuroprotective Agents/pharmacokinetics ; Proto-Oncogene Proteins c-bcl-2/metabolism
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Contributed Indexing:: Keywords: axon injury; curcumin; hippocampus; lipid-based nanodrug delivery; neurodegeneration
Substance Nomenclature:: 0 (Antioxidants)
0 (Biomarkers)
0 (Emulsions)
0 (Neuroprotective Agents)
0 (Proto-Oncogene Proteins c-bcl-2)
IT942ZTH98 (Curcumin)
Entry Date(s):: Date Created: 20201127 Date Completed: 20210108 Latest Revision: 20240330
Update Code:: 20240330
PubMed Central ID:: PMC7685369
DOI:: 10.2147/IJN.S272931
PMID:: 33244233
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Purpose: Curcumin, a polyphenol isolated from the rhizomes of turmeric, holds great potential as a neuroprotective agent in addition to its anti-inflammatory and antioxidant characteristics. The poor bioavailability and low stability of curcumin are the greatest barriers to its clinical use. This study aims to investigate the neuroprotective effect of curcumin on axonal injury, by delivering the lipophilic polyphenol to a primary hippocampal neuron culture by means of a lipid-based drug delivery system, named emulsomes.
Methods: To study neuroregeneration ex vivo, an injury model was established through single-cell laser axotomy on hippocampal neurites. Upon treatment with curcumin-loaded emulsomes (CurcuEmulsomes), curcumin and CurcuEmulsome uptake into neurons was verified by three-dimensional Z-stack images acquired with confocal microscopy. Neuron survival after axonal injury was tracked by propidium iodide (PI) and Hoechst staining. Alterations in expression levels of physiological markers, such as anti-apoptotic marker Bcl2, apoptotic marker cleaved caspase 3, neuroprotective marker Wnt3a and the neuronal survival marker mTOR, were investigated by immunocytochemistry analyses.
Results: The results indicated significant improvement in the survival rate of injured neurons upon CurcuEmulsome treatment. Bcl2 expression was significantly higher for injured neurons treated with curcumin or CurcuEmulsome. Reduction in caspase 3 expression was seen in both curcumin and CurcuEmulsome treatment, whereas there were no significant changes in Wnt3a and mTOR expression.
Conclusion: The established laser-axotomy model was proven as a reliable methodology to study neurodegenerative models ex vivo. CurcuEmulsomes delivered curcumin to primary hippocampal neurons successfully. Treated with CurcuEmulsomes, injured hippocampal neurons benefit from the neuroprotective effects of curcumin, exhibiting a higher survival rate and increased anti-apoptotic marker levels.
Competing Interests: The authors report no conflicts of interest in this work.
(© 2020 Yilmaz et al.)

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