SARS-CoV-2 genomic and subgenomic RNAs in diagnostic samples are not an indicator of active replication.

Szczegóły
Abstrakt

Tytuł:: SARS-CoV-2 genomic and subgenomic RNAs in diagnostic samples are not an indicator of active replication.
Autorzy:: Alexandersen S; Geelong Centre for Emerging Infectious Diseases, Geelong, VIC, 3220, Australia. .; Deakin University, Geelong, VIC, 3220, Australia. .; Barwon Health, University Hospital Geelong, Geelong, VIC, 3220, Australia. .
Chamings A; Geelong Centre for Emerging Infectious Diseases, Geelong, VIC, 3220, Australia.; Deakin University, Geelong, VIC, 3220, Australia.
Bhatta TR; Geelong Centre for Emerging Infectious Diseases, Geelong, VIC, 3220, Australia.; Deakin University, Geelong, VIC, 3220, Australia.
Źródło:: Nature communications [Nat Commun] 2020 Nov 27; Vol. 11 (1), pp. 6059. Date of Electronic Publication: 2020 Nov 27.
Typ publikacji:: Journal Article; Research Support, Non-U.S. Gov't
Język:: English
Imprint Name(s):: Original Publication: [London] : Nature Pub. Group
MeSH Terms:: Genome, Viral*
Virus Replication*
COVID-19/*diagnosis
COVID-19 Testing/*methods
RNA, Viral/*isolation & purification
SARS-CoV-2/*genetics
Adult ; COVID-19/virology ; Cytoplasm/virology ; Female ; High-Throughput Nucleotide Sequencing ; Host-Pathogen Interactions/genetics ; Humans ; Male ; Middle Aged ; Nasopharynx/cytology ; Nasopharynx/virology ; Oropharynx/cytology ; Oropharynx/virology ; Polymerase Chain Reaction ; SARS-CoV-2/isolation & purification ; SARS-CoV-2/pathogenicity ; Time Factors ; Young Adult
References:: Lancet. 2020 Feb 22;395(10224):565-574. (PMID: 32007145)
Nat Commun. 2021 Jan 11;12(1):267. (PMID: 33431879)
BMC Genomics. 2014 Apr 05;15:264. (PMID: 24708189)
Cell. 2020 May 14;181(4):914-921.e10. (PMID: 32330414)
Nature. 2020 May;581(7809):465-469. (PMID: 32235945)
Euro Surveill. 2017 Mar 30;22(13):. (PMID: 28382917)
Sci Rep. 2018 Jun 6;8(1):8686. (PMID: 29875375)
BMC Biotechnol. 2018 Jan 17;18(1):3. (PMID: 29343265)
Nat Microbiol. 2020 Apr;5(4):536-544. (PMID: 32123347)
Emerg Infect Dis. 2020 Nov;26(11):2701-2704. (PMID: 32749957)
Glob Chall. 2017 Jan 10;1(1):33-46. (PMID: 31565258)
Bioinformatics. 2010 Mar 15;26(6):841-2. (PMID: 20110278)
Annu Rev Virol. 2015 Nov;2(1):265-88. (PMID: 26958916)
Lancet. 2020 Feb 15;395(10223):514-523. (PMID: 31986261)
Can Vet J. 2015 Feb;56(2):149-52. (PMID: 25694663)
J Virol. 2003 Jul;77(14):7890-902. (PMID: 12829829)
Bioinformatics. 2007 Nov 1;23(21):2947-8. (PMID: 17846036)
Nature. 2020 Oct;586(7830):578-582. (PMID: 32731258)
N Engl J Med. 2020 Oct 15;383(16):1544-1555. (PMID: 32722908)
Clin Infect Dis. 2020 Nov 19;71(16):2249-2251. (PMID: 32306036)
J Cell Biol. 1988 Jun;106(6):1893-901. (PMID: 3384849)
Virology. 1993 Sep;196(1):172-8. (PMID: 8395112)
Science. 2020 Sep 11;369(6509):1395-1398. (PMID: 32763915)
Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10157-E10166. (PMID: 30297408)
Clin Infect Dis. 2020 Nov 19;71(16):2252-2254. (PMID: 32435816)
Proc Natl Acad Sci U S A. 2010 Jul 6;107(27):12257-62. (PMID: 20562343)
Lancet Infect Dis. 2020 May;20(5):533-534. (PMID: 32087114)
Science. 2020 Aug 14;369(6505):806-811. (PMID: 32434945)
Transbound Emerg Dis. 2014 Oct;61(5):397-410. (PMID: 25098383)
Sci Rep. 2017 Jun 20;7(1):3861. (PMID: 28634337)
Bioinformatics. 2009 Aug 15;25(16):2078-9. (PMID: 19505943)
Int J Infect Dis. 2020 Feb;91:264-266. (PMID: 31953166)
PLoS Pathog. 2008 May 02;4(5):e1000054. (PMID: 18451981)
J Virol. 1990 Sep;64(9):4108-14. (PMID: 2384915)
Mol Biol Evol. 2016 Jul;33(7):1870-4. (PMID: 27004904)
Viruses. 2020 Jan 13;12(1):. (PMID: 31940999)
Sci Rep. 2019 Jun 20;9(1):8906. (PMID: 31222066)
PLoS Biol. 2020 Jun 8;18(6):e3000715. (PMID: 32511245)
Sci Rep. 2019 Mar 14;9(1):4602. (PMID: 30872719)
Brief Bioinform. 2013 Mar;14(2):178-92. (PMID: 22517427)
Sci Rep. 2018 Apr 13;8(1):5980. (PMID: 29654248)
Nature. 2020 Mar;579(7798):265-269. (PMID: 32015508)
Substance Nomenclature:: 0 (RNA, Viral)
Entry Date(s):: Date Created: 20201128 Date Completed: 20201208 Latest Revision: 20240330
Update Code:: 20240330
PubMed Central ID:: PMC7695715
DOI:: 10.1038/s41467-020-19883-7
PMID:: 33247099
: Czasopismo naukowe

Full Text Finder

Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) was first detected in late December 2019 and has spread worldwide. Coronaviruses are enveloped, positive sense, single-stranded RNA viruses and employ a complicated pattern of virus genome length RNA replication as well as transcription of genome length and leader containing subgenomic RNAs. Although not fully understood, both replication and transcription are thought to take place in so-called double-membrane vesicles in the cytoplasm of infected cells. Here we show detection of SARS-CoV-2 subgenomic RNAs in diagnostic samples up to 17 days after initial detection of infection and provide evidence for their nuclease resistance and protection by cellular membranes suggesting that detection of subgenomic RNAs in such samples may not be a suitable indicator of active coronavirus replication/infection.

Informacja